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Series GSE75247 Query DataSets for GSE75247
Status Public on Jun 28, 2016
Title Mitochondrial unfolded protein response controls matrix pre-RNA processing and translation
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The mitochondrial matrix is unique in that it must integrate folding and assembly of proteins derived from nuclear and mitochondrial genomes. In C. elegans, the mitochondrial unfolded protein response (UPRmt) senses matrix protein misfolding and induces a program of nuclear gene expression, including mitochondrial chaperonins, to promote mitochondrial proteostasis. While misfolded mitochondrial matrix-localized ornithine trans-carbamylase (OTC) induces chaperonin expression, our understanding of mammalian UPRmt is rudimentary, reflecting a lack of acute triggers for UPRmt activation. This limitation has prevented analysis of the cellular responses to matrix protein misfolding and the effects of UPRmt on mitochondrial translation to control protein folding loads. Here, we combine pharmacological inhibitors of matrix-localized HSP90/TRAP1 or LON protease, which promote chaperonin expression, with global transcriptional and proteomic analysis to reveal an extensive and acute response of human cells to UPRmt. This response involved widespread induction of nuclear genes, including matrix-localized proteins involved in folding, pre-RNA processing and translation. Functional studies revealed rapid but reversible translation inhibition in mitochondria occurring concurrently with defects in pre-RNA processing due to transcriptional repression and LON-dependent turnover of the mitochondrial pre-RNA processing nuclease MRPP3. This study reveals that acute mitochondrial protein folding stress activates both increased chaperone availability within the matrix and reduced matrix-localized protein synthesis through translational inhibition, and provides a framework for further dissection of mammalian UPRmt.
Overall design triplicate experiment of 3 conditions (untreated, GTPP treatment, CDDO treatment)
Contributor(s) Münch C, Harper JW
Citation(s) 27350246
Submission date Nov 20, 2015
Last update date May 15, 2019
Contact name Christian Munch
Organization name Harvard Medical School
Department Cell Biology
Street address 240 Longwood Avenue
City Boston
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (9)
GSM1947371 DMSO sample 1
GSM1947372 DMSO sample 2
GSM1947373 DMSO sample 3
This SubSeries is part of SuperSeries:
GSE75411 Mitochondrial unfolded protein response controls matrix pre-RNA processing and translation
BioProject PRJNA302895
SRA SRP066487

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Supplementary file Size Download File type/resource
GSE75247_processed_data.txt.gz 1.4 Mb (ftp)(http) TXT
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