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Series GSE75189 Query DataSets for GSE75189
Status Public on Nov 25, 2015
Title High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The ability to perturb genes in human cells is crucial for elucidating gene function and holds great potential for finding therapeutic targets for diseases such as cancer. To extend the catalog of human core and context- dependent fitness genes, we have developed a high-complexity second-generation genome-scale CRISPR-Cas9 gRNA library and applied it to fitness screens in five human cell lines. Using an improved Bayesian analytical approach, we consistently discover 5-fold more fitness genes than were previously observed. We present a list of 1,580 human core fitness genes and describe their general properties. Moreover, we demonstrate that context-dependent fitness genes accurately recapitulate pathway-specific genetic vulnerabilities induced by known oncogenes and reveal cell-type-specific dependencies for specific receptor tyrosine kinases, even in oncogenic KRAS backgrounds. Thus, rigorous identification of human cell line fitness genes using a high-complexity CRISPR-Cas9 library affords a high-resolution view of the genetic vulnerabilities of a cell. Additional data can be found at
Overall design RNAseq of five human cell lines with Cas9 knock-ins.
Contributor(s) Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J
Citation(s) 26627737
Submission date Nov 19, 2015
Last update date May 15, 2019
Contact name Jason Moffat
Organization name University of Toronto
Department CCBR
Lab Moffat
Street address 160 College St
City Toronto
State/province ON
ZIP/Postal code M5S 3E1
Country Canada
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (5)
GSM1944747 DLD1-Cas9
GSM1944748 GBM-Cas9
GSM1944749 HeLa-Cas9
BioProject PRJNA302748
SRA SRP066424

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Supplementary file Size Download File type/resource
GSE75189_fpkm-log2-all_samples.xlsx.gz 1.1 Mb (ftp)(http) XLSX
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