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| Status |
Public on Mar 27, 2017 |
| Title |
Identification of binding sites for σ54 (RpoN) in Salmonella Typhimurium ATCC14028 [ChIP] |
| Platform organism |
Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S |
| Sample organism |
Salmonella enterica subsp. enterica serovar Typhimurium str. ATCC 14028 |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
ChIP-chip analysis of DNA specifically bound to anti-σ54 from mid-exponentially growing Salmonella enterica sv Typhimurium ATCC14028 wild type and ΔrpoN mutant cultures containing plasmid pPBHP192 expressing protein DctD250
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| Overall design |
DNA specifically bound to anti-σ54 was harvested from mid-exponentially growing Salmonella enterica sv Typhimurium ATCC14028 wild type (three biological replicates) and ΔrpoN mutant (two replicates) cultures that contain plasmid pPBHP192 (expressing protein DctD250). DNA was labeled and hybridized to an overlapping high density oligo array.
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| Contributor(s) |
Porwollik S, Karls AC, McClelland M |
| Citation missing |
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| |
| Submission date |
Nov 19, 2015 |
| Last update date |
Mar 29, 2017 |
| Contact name |
Michael McClelland |
| E-mail(s) |
mcclelland.michael@gmail.com
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| Phone |
858-336-9554
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| Organization name |
University of California, Irvine
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| Department |
Microbiology & Molecular Genetics
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| Street address |
132 Med Surge I
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| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697 |
| Country |
USA |
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| Platforms (1) |
| GPL14855 |
NIMBLE_MMCCSAL07_14028_380k [MMCCSAL07_100416_SP_CGH] |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE75180 |
Identification of regulatory activities and binding sites for σ54 (RpoN) in Salmonella Typhimurium ATCC14028 |
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| Relations |
| BioProject |
PRJNA302719 |
