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Status |
Public on Nov 03, 2007 |
Title |
HrcA Regulon in Listeria monocytogenes |
Platform organisms |
Listeria monocytogenes; Listeria innocua |
Sample organism |
Listeria monocytogenes |
Experiment type |
Expression profiling by array
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Summary |
The foodborne pathogen Listeria monocytogenes uses a number of transcriptional regulators, including the negative regulator HrcA, to control gene expression under different environmental conditions and in response to stress. Gene expression patterns of DhrcA stationary phase cells were compared to wt to identify hrcA-dependent genes. We identified 61 HrcA-dependent genes that showed significant expression ratios (adj. P < 0.05), with ≥ 1.5-fold differential expression between ΔhrcA and wt. Combined with microarray analysis, Hidden Markov Model searches show HrcA directly repress at least 8 genes. Keywords: Listeria monocytogenes, HrcA regulon, stationary phase
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Overall design |
Independent RNA isolations were performed for each growth experiment (log phase cells). For wt vs. hrcA null mutant comparisons, three biological replicates were used in competitive whole-genome microarray experiments. For each set of hybridizations, RNA from the parent strain (10403S) was hybridized to RNA from the hrcA null mutant.
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Contributor(s) |
Hu Y, Raengpradub S, Wiedmann M, Boor KJ |
Citation(s) |
17965207 |
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Submission date |
Apr 13, 2007 |
Last update date |
Mar 16, 2012 |
Contact name |
Sarita Raengpradub |
E-mail(s) |
sr266@cornell.edu
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Organization name |
Cornell University
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Department |
Food Science
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Lab |
Food Safety Lab
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Street address |
412 Stocking Hall
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City |
Ithaca |
State/province |
NY |
ZIP/Postal code |
14853 |
Country |
USA |
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Platforms (1) |
GPL5029 |
CUFSL Listeria 6K oligonuclotide array v1.0 |
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Samples (1) |
GSM182055 |
HrcA regulon, 10403S vs delta hrcA, stationary phase |
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Relations |
BioProject |
PRJNA99417 |
Supplementary file |
Size |
Download |
File type/resource |
GSE7517_RAW.tar |
1.5 Mb |
(http)(custom) |
TAR (of GPR) |
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