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Series GSE7517 Query DataSets for GSE7517
Status Public on Nov 03, 2007
Title HrcA Regulon in Listeria monocytogenes
Platform organisms Listeria monocytogenes; Listeria innocua
Sample organism Listeria monocytogenes
Experiment type Expression profiling by array
Summary The foodborne pathogen Listeria monocytogenes uses a number of transcriptional regulators, including the negative regulator HrcA, to control gene expression under different environmental conditions and in response to stress. Gene expression patterns of DhrcA stationary phase cells were compared to wt to identify hrcA-dependent genes.
We identified 61 HrcA-dependent genes that showed significant expression ratios (adj. P < 0.05), with ≥ 1.5-fold differential expression between ΔhrcA and wt. Combined with microarray analysis, Hidden Markov Model searches show HrcA directly repress at least 8 genes.
Keywords: Listeria monocytogenes, HrcA regulon, stationary phase
 
Overall design Independent RNA isolations were performed for each growth experiment (log phase cells). For wt vs. hrcA null mutant comparisons, three biological replicates were used in competitive whole-genome microarray experiments. For each set of hybridizations, RNA from the parent strain (10403S) was hybridized to RNA from the hrcA null mutant.
 
Contributor(s) Hu Y, Raengpradub S, Wiedmann M, Boor KJ
Citation(s) 17965207
Submission date Apr 13, 2007
Last update date Mar 16, 2012
Contact name Sarita Raengpradub
E-mail(s) sr266@cornell.edu
Organization name Cornell University
Department Food Science
Lab Food Safety Lab
Street address 412 Stocking Hall
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
 
Platforms (1)
GPL5029 CUFSL Listeria 6K oligonuclotide array v1.0
Samples (1)
GSM182055 HrcA regulon, 10403S vs delta hrcA, stationary phase
Relations
BioProject PRJNA99417

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7517_RAW.tar 1.5 Mb (http)(custom) TAR (of GPR)

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