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| Status |
Public on Aug 15, 2009 |
| Title |
Gene expression data from cancer mammospheres and bulk tumors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Tumorigenic breast cancer cells characterized by CD44 expression and low or undetectable CD24 levels (CD44+/CD24-/low) may be resistant to chemotherapy and therefore responsible for cancer relapse. Paired breast cancer core biopsies before and after neoadjuvant chemotherapy or lapatinib were obtained and as single cell suspensions stained using antibodies against CD24, CD44, and lineage markers, and then analyzed by flow cytometry. Mammosphere (MS) formation in culture was compared before and after treatment. Global gene expression differences between cancer cells bearing CD44+/CD24-/low cells and all other sorted cells, and between cancer MS and the primary bulk invasive cancers were analyzed. We report that CD44+/CD24-/low tumorigenic breast cancer cells were intrinsically chemoresistant ─ chemotherapy led to increased CD44+/CD24-/low cells, increased self-renewal capacity on MS assays, and enhanced tumorigeneicity in immunocompromised SCID/Beige mice. Conversely, in patients with HER2 overexpressing tumors, the EGFR/HER2 tyrosine kinase inhibitor, lapatinib decreased CD44+/CD24-/low cells, with the majority of these patients after conventional therapy achieving pathologic complete response, a validated surrogate marker for long-term survival. Gene transcription pathways that underlie chemoresistant, MS-forming CD44+/CD24-/low cells involve genes belonging to stem cell self-renewal, Wnt signaling, and early development pathways.
Keywords: two group comparison
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| Overall design |
Cells from human breast tumors were grown as mammospheres (MS).
Isolated single cell suspensions from primary breast cancers were plated onto non-adherent (polyhema-coated) plastic, counted with a hematocytometer, and 20,000 cells were then seeded into a 6-well ultra-low attachment plate supplemented with 2mL MEGM, with the addition of 2 mL of freshly unfrozen MEGM every 3-4 days. Gene expression profiles were taken of both MS and primary bulk tumors and compared with each other.
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| Contributor(s) |
Creighton C, Lewis M, Chang J |
| Citation(s) |
19666588 |
| Submission date |
Apr 13, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
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| Organization name |
Baylor College of Medicine
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| Department |
Biostatistics, Ducan Cancer Center
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| Street address |
One Baylor Plaza, Mail Stop: BCM305
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (26)
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| GSM194618 |
Cancer mammospheres, biological rep1 |
| GSM194620 |
Cancer mammospheres, biological rep2 |
| GSM194621 |
Cancer mammospheres, biological rep3 |
| GSM194622 |
Cancer mammospheres, biological rep4 |
| GSM194623 |
Cancer mammospheres, biological rep5 |
| GSM194624 |
primary breast cancer, patient 1 |
| GSM194625 |
primary breast cancer, patient 2 |
| GSM194626 |
primary breast cancer, patient 3 |
| GSM194627 |
primary breast cancer, patient 4 |
| GSM194628 |
primary breast cancer, patient 5 |
| GSM194629 |
primary breast cancer, patient 6 |
| GSM194630 |
primary breast cancer, patient 7 |
| GSM194631 |
primary breast cancer, patient 8 |
| GSM194632 |
primary breast cancer, patient 9 |
| GSM194633 |
primary breast cancer, patient 10 |
| GSM194634 |
primary breast cancer, patient 11 |
| GSM194636 |
Cancer mammospheres, biological rep6 |
| GSM194637 |
Cancer mammospheres, biological rep7 |
| GSM194639 |
Cancer mammospheres, biological rep8 |
| GSM194640 |
Cancer mammospheres, biological rep9 |
| GSM194641 |
Cancer mammospheres, biological rep10 |
| GSM194642 |
Cancer mammospheres, biological rep11 |
| GSM194643 |
Cancer mammospheres, biological rep12 |
| GSM194644 |
Cancer mammospheres, biological rep13 |
| GSM194645 |
Cancer mammospheres, biological rep14 |
| GSM194646 |
Cancer mammospheres, biological rep15 |
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| Relations |
| BioProject |
PRJNA100467 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7515_RAW.tar |
209.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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