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| Status |
Public on Apr 13, 2007 |
| Title |
Identification of LEDGF-responsive genes in Jurkat cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The LEDGF transcript from the PSIP1 gene was knocked down in Jurkat cells using RNAi technology. The resulting Jurkat-derived cell line (Jurkat-siJK2) was compared to a control cell line (wild type Jurkat) using microarray analysis. Genes identified as being modulated by LEDGF were preferential targets of HIV integration.
Keywords: effects of gene knockdown
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| Overall design |
2 cell lines were used, Jurkat-siJK2 (experimental) and wild-type Jurkat (control). 3 independent cultures of each cell line were used for independent RNA extractions, labeling reactions and array hybridizations.
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| |
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| Contributor(s) |
Wang GP, Ciuffi A, Leipzig J, Berry CC, Bushman FD |
| Citation(s) |
17545577 |
| Submission date |
Apr 12, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Gary P Wang |
| E-mail(s) |
gary.wang@uphs.upenn.edu
|
| Organization name |
University of Pennsylvania School of Medicine
|
| Department |
Microbiology
|
| Lab |
Bushman
|
| Street address |
405 Johnson Pavilion, 3610 Hamilton Walk
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA100475 |
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