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Series GSE75059 Query DataSets for GSE75059
Status Public on Mar 02, 2016
Title Transcriptional profile changes by Prox1 expression in thyroid cancer cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Human thyroid cancer cell line BCPAP was engineered for doxycyclin-mediated inducible expressoin of Prox1 and next generation sequcing was performed to study the transcriptional regulation by Prox1
Overall design BCPAP cells were infected with rTTA2 lentivirus. The resulting cell populations were then stably transfected either with pIRES-hygromycin vector (Clontech) to generate BCPAP-Ctr cells, or with Flag-PROX1-IRES-hygromycin vector to generate BCPAP-Prx cells. PROX1 expression was induced or not in BCPAP-Ctr and BCPAP-Prx cells with 0.5 µg/ml doxycycline (Dox) for 48 hours and the total RNA samples were collected for sequencing.
Contributor(s) Hong Y
Citation(s) 26609053
Submission date Nov 16, 2015
Last update date May 15, 2019
Contact name Young-Kwon Hong
Organization name University of Southern California
Street address 1450 Biggy St. NRT6501
City Los Angeles
State/province CA
ZIP/Postal code 90033
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (4)
GSM1941568 BCPAP-Ctr_PBS
GSM1941569 BCPAP-Ctr_Dox
GSM1941570 BCPAP-Prx_PBS
BioProject PRJNA302305
SRA SRP066260

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Supplementary file Size Download File type/resource
GSE75059_BCPAP-CtrplusPBS_filtered.merged.genes.fpkm_tracking.gz 493.2 Kb (ftp)(http) FPKM_TRACKING
GSE75059_BCPAP-PrxplusPBS_filtered.merged.genes.fpkm_tracking.gz 490.9 Kb (ftp)(http) FPKM_TRACKING
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Raw data are available in SRA
Processed data are available on Series record

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