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Series GSE74981 Query DataSets for GSE74981
Status Public on Dec 01, 2016
Title Next generation sequencing to elucidate the novel function of RORC (RORgamma) in breast cancer
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Exploring the novel role of RORC (RORgamma) in breast cancer, utilizing NEXTseq with genetic gain and loss of function and pharmacological treatment.
Overall design For loss of function, control-siRNA or RORC-siRNA was transfected for 48h in three cell lines (MCF-7, T-47D and MDA-MB-231). For gain of function, CMV-empty or CMV-RORC was transfected for 48h in MDA-MB-231 cells. Furthermore, the selective RORC antagonist, SR2211 was utilized. MCF-7 cells were treated either DMSO or SR2211 (5uM) for 24h. Total RNA was extracted with the RNeasy kit. NEXTseq was performed for transcriptome analysis.
Contributor(s) Oh TG, Muscat GE
Citation(s) 27211549
Submission date Nov 13, 2015
Last update date May 27, 2020
Contact name Tae Gyu Oh
Organization name Institute for Molecular Bioscience
Street address 306 Carmody Rd
City St Lucia
ZIP/Postal code 4072
Country Australia
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (30)
GSM1939609 MCF-7_CONT_1
GSM1939610 MCF-7_CONT_2
GSM1939611 MCF-7_CONT_3
BioProject PRJNA302118
SRA SRP066204

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74981_RAW.tar 6.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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