GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE74960 Query DataSets for GSE74960
Status Public on Feb 22, 2016
Title Role of the histone demethylase KDM2B in hematopoietic homeostasis and malignancies [Kras background mouse gene expression]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Development of the hematopoietic system is dynamically controlled by the interplay of transcriptional and epigenetic networks to determine cellular identity. Those networks are critical for homeostasis and frequently dysregulated in leukemias. We identified histone demethylase Kdm2b as a critical regulator of definitive hematopoiesis and lineage specification of hematopoietic stem and progenitor cells (HSPCs). RNA sequencing in murine HSPCs and genome-wide chromatin immunoprecipitation studies in human leukemias revealed that Kdm2b regulates differentiation, lineage choice, cytokine signaling, and quiescence.
Comparison of gene expression in wild-type and knockout HSPCs
Overall design We analyzed lineage depleted cells isolated from bone marrow from mice that express oncogenic Kras in the context of knockout and overexpression of Kdm2b in Affymetrxi GeneChip® Mouse Gene 2.0 ST Array
Contributor(s) Tzatsos A, Kai Y
Citation(s) 26808549
Submission date Nov 12, 2015
Last update date Feb 22, 2016
Contact name Alexandros Tzatsos
Phone 2029949104
Organization name George Washington University
Department GW Cancer Center
Lab Cancer Epigenetics Laboratory
Street address 800 22nd St NW SEH 8850
City Washington
State/province DC
ZIP/Postal code 20052
Country USA
Platforms (1)
GPL18233 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [probe set (exon) version]
Samples (8)
GSM1939041 PA2681_WT_1
GSM1939042 PA2681_WT_2
GSM1939043 PA2685_KO_1
This SubSeries is part of SuperSeries:
GSE70729 Role of the histone demethylase KDM2B in hematopoietic homeostasis and malignancies
BioProject PRJNA302012

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74960_RAW.tar 93.9 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap