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Series GSE74771 Query DataSets for GSE74771
Status Public on Oct 23, 2018
Title Single-base resolution methylome of 5-methylcytosine in mammalian transcriptome
Organisms Homo sapiens; Mus musculus
Experiment type Other
Summary mRNAs of human and mouse cell lines were purified using oligo (dT)-conjugated magnetic beads followed by RiboMinus treatement. mRNAs were fragmented to ~100 nt and treated by Sodium bisulfite solution (pH 5.1) containing Hydroquinone. Bisulfite treated mRNAs were reverse transcribed to cDNA using ACT random hexamer primers. The cDNAs were subjected to libraries construction using KAPA Stranded mRNA-Seq Kit (KAPA) and performed sequencing on HiSeq2000/3000 (Illumina) in pair-end mode, creating reads with a length of 101 bp. Sequencing chemistry v2 (Illumina) was used and samples were multiplexed in two samples per lane.
Overall design Explore the single-base resolution methylome of 5-Methylcytosine in mammalian transcriptome
Contributor(s) Yang Y, Yang Y, Sun B
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Submission date Nov 06, 2015
Last update date May 15, 2019
Contact name Bao-Fa Sun
Organization name Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
Street address Da-Tun Road
City Beijing
ZIP/Postal code 100101
Country China
Platforms (4)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (31)
GSM1932918 siCTRL_HeLa_rep1BS
GSM1932919 siCTRL_HeLa_rep2BS
GSM1932920 siNSUN2_HeLa_rep1BS
BioProject PRJNA301456
SRA SRP065906

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74771_RAW.tar 2.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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