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Series GSE74748 Query DataSets for GSE74748
Status Public on May 03, 2016
Title Effect of Cited2 knockdown on global transcript expression in Rcho-1 cell differentiation
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary We had previously discovered that the transcription factor Cited2 was highly induced during trophoblast differentiation. In this study, we used an lentiviral shRNA strategy to decrease Cited2 expression in Rcho-1 trophoblast cells. A RNA-seq approach was used to determine global transcript differences inRcho-1 knockdown cells compared to control cells.
Overall design Rcho-1 cells transduced with control shRNAs were used as controls. Cells transduced with shRNAs targetingCited2 were used as treatment.Cells were differentiated for 8 days and the analyses were done.
Contributor(s) Dhakal P, Imakawa K, Soares MJ
Citation(s) 26917451
Submission date Nov 06, 2015
Last update date May 15, 2019
Contact name Pramod Dhakal
Phone 913-588-5690
Organization name University of Kansas Medical Center
Department Pathology and Laboratory Medicine
Street address 3901 Rainbow Boulevard
City Kansas City
State/province Kansas
ZIP/Postal code 66160
Country USA
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (6)
GSM1931651 Control 1
GSM1931652 Control 2
GSM1931653 Control 3
BioProject PRJNA301403
SRA SRP065879

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Supplementary file Size Download File type/resource
GSE74748_RAW.tar 24.1 Mb (http)(custom) TAR (of XLSX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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