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Series GSE74710 Query DataSets for GSE74710
Status Public on Nov 06, 2015
Title Transient transfection of miR-449 family in the hepatocellular carcinoma cell line HLE
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In this study, we comparatively analyzed the members of the miR-449 family (miR-449a, miR-449b, and miR-449c) with regards to their target genes and functional effects in hepatocellular carcinoma (HCC). Microarray analysis after transient transfection of miR-449a, miR-449b, and/or miR-449c in the HCC cell line HLE identified putative target genes of miR-449a, miR-449b, and miR-449c.
 
Overall design For transient overexpression of miRNAs, the HCC cell line HLE was transfected with 50 nM Allstars Negative Control or miScript miRNA mimics. MiRNA mimics for miR-449a, miR-449b, and miR-449c were either single transfected or cotransfected in equimolar amounts (16.7 nM each). Global mRNA expression profiling was performed utilizing pooled RNA of three biological replicates per microarray and two microarrays per condition.
 
Contributor(s) Sandbothe M, Buurman R, Greiwe L, Vajen B, Schäffer V, Eilers M, Illig T, Schlegelberger B, Skawran B
Citation(s) 28088579
Submission date Nov 05, 2015
Last update date Jan 09, 2018
Contact name Maria Sandbothe
Organization name Hannover Medical School
Street address Carl-Neuberg-Str. 1
City Hannover
ZIP/Postal code 30625
Country Germany
 
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (10)
GSM1930719 HLE_NC_1
GSM1930720 HLE_NC_2
GSM1930721 HLE_449a_1
Relations
BioProject PRJNA301220

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74710_RAW.tar 30.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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