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Series GSE74582 Query DataSets for GSE74582
Status Public on Nov 03, 2015
Title Small nucleolar RNA and microRNA expression data from an MS patient's and a healthy control's PBMC cultures stimulated with phytohemagglutinin.
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Multiple sclerosis is a common inflammatory and degenerative disease that causes neurological disability. It affects young adults and its prevalence is higher in women. The most common form is manifested as a series of acute episodes of neurological disability (relapses) followed by a recovery phase (remission). Recently, non-coding RNAs have emerged as new players in transcriptome regulation, and in turn, they could have a significant role in MS pathogenesis. In this context, our aim was to investigate the involvement of microRNAs and snoRNAs in the relapse-remission dynamics of MS in peripheral blood leucocytes, to shed light on the molecular and regulatory mechanisms that underlie this complex process. With this approach, we found that a subset of small non-coding RNAs (sncRNA) is altered in relapse and remission, revealing unexpected opposite changes that are sex dependent. Furthermore, we found that a relapse-related miRNA signature regulated general metabolism processes in leucocytes, and miRNA altered in remission are involved in the regulation of innate immunity. We observed that sncRNA dysregulation is different in relapse and remission leading to differences in transcriptome regulation, and that this process is sex dependent. In conclusion, relapse and remission have a different molecular background in men and women.
Overall design PBMC samples from a MS patient and a healthy control cultured with and without stimulation with phytohemagglutinin.
Contributor(s) Muñoz-Culla M, Otaegui D
Citation(s) 26831009
Submission date Nov 02, 2015
Last update date May 02, 2017
Contact name Maider Muñoz-Culla
Organization name Biodonostia Institute
Street address Paseo Dr.Begiristain s/n
City San Sebastian
ZIP/Postal code 20014
Country Spain
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (4)
GSM1923349 cultured PBMC from MS patient, no stimulated
GSM1923350 cultured PBMC from MS patient stimulated with PHA
GSM1923351 cultured PBMC from healthy control, no stimulated
This SubSeries is part of SuperSeries:
GSE74586 SncRNA (microRNA & snoRNA) expression in leucocytes from multiple sclerosis relapse and remission and cultured peripheral blood mononuclear cells
BioProject PRJNA300874

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Supplementary file Size Download File type/resource
GSE74582_RAW.tar 3.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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