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Series GSE74547 Query DataSets for GSE74547
Status Public on Jan 01, 2016
Title ETO Family Protein Mtgr1 Mediates Prdm14 Functions in Stem Cell Maintenance
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Prdm14 is a sequence-specific transcriptional regulator of embryonic stem cell (ESC) pluripotency and primordial germ cell (PGC) formation. It exerts its function, at least in part, through repressing genes associated with epigenetic modification and cell differentiation. Here, we show that this repressive function is mediated through an ETO-family co-repressor Mtgr1, which tightly binds to the pre-SET/SET domains of Prdm14 and co-occupies its genomic targets in mouse ESCs. Structure-guided point mutants abrogated the Prdm14-Mtgr1 association and disrupted Prdm14's function in mESC gene expression and PGC formation in vitro. Altogether, our work uncovers the molecular mechanism underlying Prdm14-mediated repression.
Overall design Examination of Prdm14 and Mtgr1 occupancy by ChIP-seq and effects on gene expression in mouse embryonic stem cells
Contributor(s) Nady N, Swigut T, Wysocka J
Citation(s) 26523391
Submission date Oct 30, 2015
Last update date May 15, 2019
Contact name Natalie Nady
Organization name Stanford
Department Chemical and Systems Biology
Lab Wysocka
Street address 265 Campus Dr.
City Stanford
State/province CA
ZIP/Postal code 94303
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (26)
GSM1922468 wt_Mtgr1_ChIPseq
GSM1922469 Prdm14null_G6_Mtgr1_ChIPseq
GSM1922470 Prdm14null_F10_Mtgr1_ChIPseq
BioProject PRJNA300681
SRA SRP065555

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Supplementary file Size Download File type/resource
GSE74547_RAW.tar 451.2 Mb (http)(custom) TAR (of TXT, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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