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Series GSE74457 Query DataSets for GSE74457
Status Public on Feb 01, 2016
Title A Dual Molecular Analog Tuner for Dissecting Mammalian Protein Function
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Loss-of-function studies are fundamental for dissecting gene function. Yet, methods to rapidly and effectively perturb genes in mammalian cells are scarce. We present a novel system, deliverable with only two lentiviral vectors, which enables simultaneous control over two different proteins in the same cell. By harnessing the plant auxin and jasmonate hormone-induced degradation pathways, combined with RNA interference, this system allows constitutive depletion of two endogenous proteins and their replacement with two exogenous proteins whose degradation is rapidly and reversibly induced by external ligands, representing a dual analog molecular tuner. Focusing on NANOG, CHK1 and NOTCH1 in embryonic stem cells and p53 in cancer cells we have validated the efficiency, rapidity, reversibility, titratability and multiplicity of the engineered tuners, and demonstrated their potential to facilitate previously-unfeasible experimental approaches and to generate novel biological insights.
Overall design For mRNA-Seq preparation, coronatine/DMSO treated cells were collected.
Contributor(s) Brosh R, Waghray A, Lemischka IR
Citation(s) 27230261
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 GM078465 Global & Systems Level Analyses of Cell Fate Regulation MOUNT SINAI SCHOOL OF MEDICINE IHOR R LEMISCHKA
Submission date Oct 28, 2015
Last update date May 15, 2019
Contact name avinash waghray
Organization name Massachussets General Hospital
Department Center for Regenerative Medicine
Lab Rajagopal
Street address 185 Cambridge Street, CPZN Room 4254A
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (4)
GSM1921000 DMSO_A
GSM1921001 Cor_A
GSM1921002 DMSO_B
BioProject PRJNA300451
SRA SRP065451

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Supplementary file Size Download File type/resource
GSE74457_genes_fpkm.xlsx.gz 1.9 Mb (ftp)(http) XLSX
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