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Series GSE74451 Query DataSets for GSE74451
Status Public on Aug 04, 2016
Title Identification of a novel risk factor for intracranial aneurysms in ADPKD using iPSC models [Agilent]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Cardiovascular complications are the leading cause of death in autosomal dominant polycystic kidney disease (ADPKD), and intracranial aneurysm (ICA) causing subarachnoid hemorrhage is among the most serious complications. The diagnostic and therapeutic strategies for ICAs in ADPKD have not been fully established. We here generated induced pluripotent stem cells (iPSCs) from seven ADPKD patients, including four with ICAs. The vascular cells differentiated from ADPKD-iPSCs showed altered Ca2+ entry and gene expression profiles compared with those from control-iPSCs. We found that the expression level of a metalloenzyme gene, matrix metalloproteinase (MMP) 1, was specifically elevated in the iPSC-derived endothelia from ADPKD patients with ICAs. Furthermore, we confirmed a statistically significant correlation between the serum MMP1 levels and the development of ICAs in 354 ADPKD patients, indicating that the serum MMP1 levels may be a novel risk factor and become more beneficial when combined with other risk factors. These results suggest that cellular disease models with ADPKD-specific iPSCs can be used to study the disease mechanisms and to identify novel disease-related molecules or risk factors.
 
Overall design The gene expression profiles of vascular endothelia and smooth muscle cells derived from control- and ADPKD-iPSCs were analyzed. Seven control-iPSC derived endothelial cells (ECs), seven ADPKD-iPSC derived ECs, ten control-iPSC derived vascular smooth muscle cells (SMCs), and seven ADPKD-iPSC derived SMCs were analyzed.
 
Contributor(s) Ameku T, Shiota F, Kitaoka F, Amano N, Nakamura M, Watanabe A, Osafune K
Citation(s) 27418197
Submission date Oct 28, 2015
Last update date Jan 09, 2018
Contact name Akira Watanabe
E-mail(s) a.watanabe@cyberomix.com
Organization name CyberomiX Inc
Street address Rm504, Miyako Bldg, 233, Isa-cho, kamigyo-ku
City Kyoto
State/province Kyoto
ZIP/Postal code 602-8407
Country Japan
 
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (31)
GSM1920920 TIG114 4F1_iPSC derived endothelial cells_rep1
GSM1920921 TIG118 4F1_iPSC derived endothelial cells_rep1
GSM1920922 TIG119 4F1_iPSC derived endothelial cells_rep1
This SubSeries is part of SuperSeries:
GSE74453 Identification of a novel risk factor for intracranial aneurysms in ADPKD using iPSC models
Relations
BioProject PRJNA300447

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74451_RAW.tar 94.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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