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Series GSE74450 Query DataSets for GSE74450
Status Public on Sep 16, 2016
Title Direct Isolation and Characterization of Human Nephron Progenitors.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Mature nephrons originate from a small population of uninduced nephrogenic progenitor cells (NPs) within the cap mesenchyme. These cells are characterized by the coexpression of SIX2 and CITED1. Many studies on mouse models as well as on human pluripotent stem cells have advanced our knowledge of NPs, but very little is known about this population in humans, since it is exhausted before birth and strategies for its direct isolation are still limited. Here we report an efficient protocol for direct isolation of human NPs without genetic manipulation or stepwise induction procedures. With the use of RNA-labeling probes, we isolated SIX2+CITED1+ cells from human fetal kidney for the first time. We confirmed their nephrogenic state by gene profiling and evaluated their nephrogenic capabilities in giving rise to mature renal cells. We also evaluated the ability to culture these cells without complete loss of SIX2 and CITED1 expression over time. In addition to defining the gene profile of human NPs, this in vitro system facilitates studies of human renal development and provides a novel tool for renal regeneration and bioengineering purposes
Overall design Single cell suspensions from 3 freshly isolated human fetal kidneys ( 17 weeks of gestation) were labelled overnight with RNA probes for SIX2 and CITED1 and sorted to obtain a 1) double positive population and2) a negative fraction. Long term goal is to investigate the transcription profile that differentiate positive versus negative selections.
Web link
Contributor(s) Perin L, Da Sacco S, Thornton ME
Citation(s) 28191781
Submission date Oct 28, 2015
Last update date May 15, 2019
Contact name Brendan H Grubbs
Organization name University of Southern California
Department Obstetrics and Gynecology
Lab Grubbs
Street address 4661 W. Sunset Blvd.
City Los Angeles
State/province CA
ZIP/Postal code 90027
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (8)
GSM1920902 human fetal kidney isolate GW 17 positive (paired-end)
GSM1920903 human fetal kidney isolate GW 17 positive (single)
GSM1920904 human fetal kidney isolate GW 17 negative (paired-end)
BioProject PRJNA300441
SRA SRP065449

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Supplementary file Size Download File type/resource
GSE74450_01Sep15_hFK_topTable_DE_rpkm_annotated.xls.gz 4.2 Mb (ftp)(http) XLS
GSE74450_02Sep15_hAF_empirical_DE_rpkm_annotated.xls.gz 3.7 Mb (ftp)(http) XLS
GSE74450_gencode_v22_ERCC.gtf.gz 41.7 Mb (ftp)(http) GTF
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Raw data are available in SRA
Processed data are available on Series record

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