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Series GSE74240 Query DataSets for GSE74240
Status Public on Aug 22, 2017
Title Effects of isoxazole (ISX) on long-term cultured human islets
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Novel strategies are needed to modulate β-cell differentiation and function as potential β-cell replacement or restorative therapies for diabetes. We previously demonstrated that small molecules based on the isoxazole scaffold drive neuroendocrine phenotypes. The nature of the effects of isoxazole compounds on β cells was incompletely defined. We find that isoxazole largely induced genes that support neuroendocrine and β-cell phenotypes, and suppressed a set of genes important for proliferation. Isoxazole alters β-cell metabolites and protects glucose-responsive signaling pathways under lipotoxic conditions. Finally, we show that isoxazole improves glycemia in a mouse model of β-cell regeneration. Isoxazole is a prime candidate for altering cell fate in different contexts.
Overall design We analyzed 3 pairs of long-term cultured human islet cells treated with either DMSO or ISX (20uM) for 7 days prior to RNA extraction using the Affymetrix GeneChip Human Transcriptome Array 2.0 platform. Data was analyzed in the Affymetrix Expression Console and Transcriptome Analysis Console v2.0. No technical replicates were performed.
Contributor(s) Kalwat MA, Huang Z, Wichaidit C, McGlynn K, Savoia C, Dioum E, Schneider JW, Hutchison MR, Cobb MH
Citation(s) 26828310
Submission date Oct 21, 2015
Last update date Aug 22, 2017
Contact name Michael A Kalwat
Organization name Indiana Biosciences Research Institute
Department Lilly Diabetes Center of Excellence
Lab Kalwat Lab
Street address 1210 Waterway Blvd Suite 2000
City Indianapolis
State/province IN
ZIP/Postal code 46077
Country USA
Platforms (1)
GPL17585 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [probe set (exon) version]
Samples (6)
GSM1915208 DMSO n1
GSM1915209 ISX n1
GSM1915210 DMSO n2
BioProject PRJNA299592

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Supplementary file Size Download File type/resource
GSE74240_RAW.tar 207.2 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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