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Series GSE74182 Query DataSets for GSE74182
Status Public on Mar 07, 2016
Title Microarray analysis of regulatory and inflammatory macrophages differentiated with glycolysis inhibitor
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The gene expression patterns of macrophages were analyzed to understand how glycolysis inhibitor affects macrophage differentiation. Human monocytes derived from peripheral blood were cultured in vitro in the presence of macrophage colony-stimulating factor (M-CSF) to yield regulatory macrophages (M-Mφs), or M-CSF plus interferon-gamma to yield inflammatory macrophages (Mγ-Mφs). 2-deoxy-D-glucose (2-DG), a glycolysis inhibitor, was added during macrophage differentiation. Gene expression patterns of monocytes, M-Mφs and Mγ-Mφs diffrentiated with or without 2-DG were analyzed by microarray analysis.
Overall design Gene expression patterns of monocytes, M-Mφs and Mγ-Mφs differentiated with or without 3 mM 2-DG for 6-days were analyzed. For each condition, equal amounts of RNA from 3 individuals were mixed and provided for microarray analysis.
Contributor(s) Suzuki H, Hisamatsu T, Chiba S, Mori K, Kitazume MT, Shimamura K, Nakamoto N, Matsuoka K, Ebinuma H, Naganuma M, Kanai T
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Submission date Oct 20, 2015
Last update date Jan 09, 2018
Contact name Hiroaki Suzuki
Organization name Ajinomoto Pharmaceuticals Co., LTD.
Street address 1-1, Suzuki-cho, Kawasaki-ku
City Kawasaki-shi
State/province Kanagawa
ZIP/Postal code 210-8681
Country Japan
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (5)
GSM1912860 monocytes
GSM1912861 M-Mφs_wo2DG
GSM1912862 Mγ-Mφs _wo2DG
BioProject PRJNA299272

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74182_RAW.tar 15.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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