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Series GSE74097 Query DataSets for GSE74097
Status Public on Nov 19, 2015
Title Silencio/CG9754 connects the Piwi-piRNA complex to the cellular heterochromatin machinery
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary The repression of transposable elements in eukaryotes often involves their transcriptional silencing via targeted chromatin modifications. In animal gonads, nuclear Argonaute proteins of the PIWI-clade complexed with small guide RNAs (piRNAs) serve as sequence specificity determinants in this process. How binding of nuclear PIWI-piRNA complexes to nascent transcripts orchestrates heterochromatin formation and transcriptional silencing is unknown. Here, we characterize CG9754/Silencio as an essential piRNA pathway factor that is required for Piwi-mediated transcriptional silencing in Drosophila. Ectopic targeting of Silencio to RNA or DNA is sufficient to elicit silencing independent of Piwi and known piRNA pathway factors. Instead, Silencio requires the H3K9 methyl-transferase Eggless/SetDB1 for its silencing ability. In agreement with this, SetDB1 but not Su(var)3-9 is required for Piwi-mediated transcriptional silencing genome-wide. Due to its interaction with the target-engaged Piwi-piRNA complex we suggest that Silencio acts as linker between the sequence specificity factor Piwi and the cellular heterochromatin machinery.
 
Overall design Impact of depletion of CG9754/Silencio, HP1a, SetDB1, Su(var)3-9, G9a on transcription and the H3K9m3 patterns in ovarian somatic cells (OSC)
 
Contributor(s) Sienski G, Brennecke J
Citation(s) 26494711
Submission date Oct 16, 2015
Last update date May 15, 2019
Contact name Grzegorz Sienski
E-mail(s) grzegorz.sienski@imba.oeaw.ac.at
Organization name IMBA-Institute of Molecular Biotechnology of Austrian Academy of Sciences
Street address Dr. Bohr-Gasse 3
City Vienna
ZIP/Postal code 1030
Country Austria
 
Platforms (1)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
Samples (46)
GSM1914726 mRNA-seq from CG9754-KD siRNA #1
GSM1914727 mRNA-seq from CG9754-KD siRNA #2
GSM1914728 mRNA-seq from G9a-KD siRNA #1 + SetDB1-KD siRNA #2
Relations
BioProject PRJNA299586
SRA SRP065203

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Supplementary file Size Download File type/resource
GSE74097_RAW.tar 3.9 Gb (http)(custom) TAR (of BEDGRAPH, BW)
GSE74097_smallRNA_IP_TEs.xlsx 65.3 Kb (ftp)(http) XLSX
GSE74097_smallRNA_cluster_counts.xlsx 50.8 Kb (ftp)(http) XLSX
SRA Run SelectorHelp
Processed data are available on Series record
Processed data provided as supplementary file
Raw data are available in SRA

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