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Series GSE74017 Query DataSets for GSE74017
Status Public on Oct 18, 2016
Title Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a (ChIP-Seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Unlike clustered HOX genes, the role of non-clustered homeobox gene family members in hematopoiesis and leukemogenesis has not been extensively studied. Here we find that the Hematopoietically-expressed Homeobox gene, Hhex, is overexpressed in acute myeloid leukemia (AML) and is essential for the initiation and propagation of MLL-ENL induced AML, but dispensable for normal myelopoiesis, indicating a specific requirement for Hhex for leukemic growth. Loss of Hhex leads to upregulated expression of the tumor suppressors p16INK4a and p19ARF, which are required for growth arrest and myeloid differentiation following Hhex deletion. Mechanistically, we show that Hhex binds to the Cdkn2a locus and is required for H3K27Me3-mediated repression of this locus. Thus, Hhex is a novel therapeutic target that is specifically required for AML stem cells to repress tumor suppressor pathways and enable continued self-renewal.
Overall design Genome-wide profiling of histone modification and Hhex binding in leukemia cells.
Contributor(s) Shields BJ, Shi W, Garnham AL, Smyth GK, McCormack MP
Citation(s) 26728554
Submission date Oct 14, 2015
Last update date May 15, 2019
Contact name Wei Shi
Organization name The Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Street address 1G Royal Parade
City Parkville
State/province Victoria
ZIP/Postal code 3050
Country Australia
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (9)
GSM1908213 ChIP-seq H3K27Me3 fl
GSM1908214 ChIP-seq H3K27Me3 D
GSM1908215 ChIP-seq H3K27Me3 D Hhex F
This SubSeries is part of SuperSeries:
GSE74019 Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a
BioProject PRJNA298747
SRA SRP064810

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74017_RAW.tar 1.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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