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Series GSE73982 Query DataSets for GSE73982
Status Public on Jun 23, 2016
Title A Primate lncRNA Mediates Notch Signaling During Neuronal Development by Sequestering miRNA [SHSY5Y cells]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Long non-coding RNAs (lncRNAs) are a diverse category of transcripts with poor conservation and have expanded greatly in primates, particularly in their brain. We identified a lncRNA, which has acquired 16 microRNA response elements (MREs) for miR-143-3p in the Catarrhini branch of primates. This lncRNA termed LncND (neuro-development) gets expressed in neural progenitor cells and then declines in mature neurons. Binding and release of miR-143-3p, by LncND, can control the expression of Notch. Its expression is highest in radial glia cells in the ventricular and outer subventricular zones of human fetal brain. Down-regulation of LncND in neuroblastoma cells reduced cell proliferation and induced neuronal differentiation, an effect phenocopied by miR-143-3p over-expression and supported by RNA-seq analysis. These findings support a role for LncND in miRNA-mediated regulation of Notch signaling in the expansion of the neural progenitor pool of primates and hence contributing to the rapid growth of the cerebral cortex.
Overall design SHSY5Y cells treated either with miR-143-3p mimic or 100 nM of siRNA specific for LncND were sequenced on NextSeq500 platform. Scrambled siRNA or miRNA sequences were used as a negative control.
Contributor(s) Zhou H, Rani N, Kosik K
Citation(s) 27263970
Submission date Oct 13, 2015
Last update date May 15, 2019
Contact name Kenneth S. Kosik
Organization name UCSB
Department NRI
Lab Kosik
Street address UC Santa Barbara
City Santa Barbara
State/province California
ZIP/Postal code 93106
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (10)
GSM1907077 NR_mir-143-1
GSM1907078 NR_mir-143-2
GSM1907079 NR_mir-ctrl-1
This SubSeries is part of SuperSeries:
GSE74210 A Primate lncRNA Mediates Notch Signaling During Neuronal Development by Sequestering miRNA
BioProject PRJNA298642
SRA SRP064761

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Supplementary file Size Download File type/resource
GSE73982_lnc-filtered.merged.genes.fpkm.txt.gz 787.2 Kb (ftp)(http) TXT
GSE73982_mir-filtered.merged.genes.fpkm.txt.gz 634.8 Kb (ftp)(http) TXT
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