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Series GSE73900 Query DataSets for GSE73900
Status Public on May 04, 2016
Title Gene Regulation by microRNA-16 forced expression in ErbB-2 positive murine breast cancer cells.
Organism Mus musculus
Experiment type Expression profiling by array
Summary miR-16 is a potent tumor suppressor in ErbB-2 positive breast cancer. The primary objetive of this study was to identify previously uncharacterized putative mRNA targets of miR-16 in ErbB-2 postitive breast cancer cells. To achieve our objective we studied the effects of exogenously expressed miR-16 on the gene expression profile of primary cultures of ErbB-2 positive murine breast cancer tumors. The C4HD tumor line displays high levels of estrogen receptor and progesterone receptor, overexpresses ErbB-2 and ErbB-3, exhibits low ErbB-4 levels, and lacks EGF-R expression.
Overall design Primary cultures of the C4HD murine breast tumor were transfected with a miR-16 precursor (pre-miR-16) or a scrambled sequence (pre-miR-Control) that does not form any known mammalian miRNA. Three biological replicates were obtained for each experimental condition.
Contributor(s) Elizalde PV, Cidlowski JA, Venturutti L, Rivas MA
Citation(s) 27157613
Submission date Oct 09, 2015
Last update date Jan 12, 2017
Contact name Patricia Virginia Elizalde
Phone +54 11 4783-2869
Organization name Instituto de Biología y Medicina Experimental
Lab Molecular Mechanisms of Carcinogenesis
Street address Vuelta de Obligado 2490
City Ciudad Autónoma de Buenos Aires
State/province Ciudad Autónoma de Buenos Aires
ZIP/Postal code C1428ADN
Country Argentina
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (6)
GSM1905527 C4HD_premiRCtrl_Rep1
GSM1905528 C4HD_premiRCtrl_Rep2
GSM1905529 C4HD_premiRCtrl_Rep3
BioProject PRJNA298360

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Supplementary file Size Download File type/resource
GSE73900_RAW.tar 54.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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