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Series GSE73792 Query DataSets for GSE73792
Status Public on Jan 01, 2016
Title Comparison of RNA profiling between wild type, med20∆, rrp6∆ and med20∆/rrp6∆ in Schizosaccharomyces pombe
Organism Schizosaccharomyces pombe
Experiment type Expression profiling by high throughput sequencing
Summary Mediator is a co-regulator of RNA polymerase II (Pol II), transducing signals from regulatory elements and transcription factors to the general transcription machinery at the promoter. We here demonstrate that Med20 influences ribosomal protein expression in fission yeast. In addition, loss of Med20 leads to an accumulation of aberrant readthrough tRNA transcripts. The aberrant transcripts are polyadenylated and targeted for degradation by the exosome.  Similarly, other specialized RNA molecules, such as snRNA, snoRNA and rRNA are also accumulated in the absence of Med20. We suggest that fission yeast Mediator takes part in a regulatory pathway of Pol III transcripts.
Overall design RNA-sequencing of wt, med20∆, rrp6∆, med20∆/rrp6∆ strains using wt as control. Each strain was sequenced using three biological replicas. The results were analyzed for differential expression.
Contributor(s) Carlsten JO, Zhu X, López MD, Samuelsson T, Gustafsson CM
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Submission date Oct 06, 2015
Last update date May 15, 2019
Contact name Jonas Carlsten
Organization name University of Gothenburg
Department Biomedicin
Lab Claes Gustafsson
Street address Medicinaregatan 9A
City Göteborg
ZIP/Postal code 413 91
Country Sweden
Platforms (1)
GPL13988 Illumina HiSeq 2000 (Schizosaccharomyces pombe)
Samples (12)
GSM1902805 WT A
GSM1902806 WT B
GSM1902807 WT C
BioProject PRJNA297893
SRA SRP064533

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Supplementary file Size Download File type/resource
GSE73792_DESeq_med20-wt.xls.gz 704.9 Kb (ftp)(http) XLS
GSE73792_DESeq_med20_rrp6-wt.xls.gz 736.9 Kb (ftp)(http) XLS
GSE73792_DESeq_rrp6-wt.xls.gz 717.1 Kb (ftp)(http) XLS
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