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Series GSE73759 Query DataSets for GSE73759
Status Public on Sep 01, 2016
Title JNK1 Ablation in Mice Confers Long-term Metabolic Protection from Diet-Induced Obesity at the Cost of Increased Skin Oxidative Damage
Organism Mus musculus
Experiment type Expression profiling by array
Summary Obesity and insulin resistance are associated with oxidative stress, which may be implicated in their progression. The kinase JNK1 emerged as a promising drug target for the treatment of obesity and type-2 diabetes. However, JNK1 is a key mediator of the oxidative stress response, promoting either cell dead or survival depending on magnitude and context of its activation. Furthermore, JNK inactivation shortens lifespan in drosophila and c. elegans. To learn on the safety and efficacy of long-term JNK inhibition in vertebrates, we investigated mice lacking JNK1 (JNK1-/-) exposed over a long period to an obesogenic high-fat diet (HFD).
JNK1-/- mice chronically fed an HFD developed more skin oxidative damage because of reduced catalase expression, but also showed sustained protection from obesity, adipose tissue inflammation, steatosis, and insulin resistance, paralleled by decreased oxidative damage in fat and liver. We conclude that JNK1 is a relatively safe drug target for obesity-related diseases.
Overall design RNA was collected from liver, skin and epididymal fat tissues from JNK1 KO mice and WT mice fed in high fat diet. Each condition was run in quadruplicate
Contributor(s) Becattini B, Zani F, Radici L, Breasson L, D'Agostino VG, Choo M, Provenzani A, Park JM, Solinas G, Fedrizzi T, Pancher M
Citation(s) 27230858
Submission date Oct 06, 2015
Last update date Jan 12, 2017
Contact name Alessandro Provenzani
Phone +390461283094
Organization name University of Trento
Department CIBIO
Lab Laboratory of Genomic Screening
Street address via delle regole 101
City Trento
ZIP/Postal code 38100
Country Italy
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (21)
GSM1902253 Skin JNK1 Knock-out rep1
GSM1902254 Skin JNK1 Knock-out rep2
GSM1902255 Skin JNK1 Knock-out rep3
BioProject PRJNA297836

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Supplementary file Size Download File type/resource
GSE73759_RAW.tar 193.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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