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Series GSE73511 Query DataSets for GSE73511
Status Public on Jun 10, 2016
Title Impact of a heterotroph, Alteromonas MIT1002, on the transcriptome of Prochlorococcus NATL2A
Organisms Alteromonas macleodii; Prochlorococcus marinus str. NATL2A
Experiment type Expression profiling by high throughput sequencing
Summary We examine how the transcriptome of Prochlorococcus strain NATL2A changes in the presence of a naturally co-occurring heterotroph, Alteromonas macleodii MIT1002. Significant changes in the Prochlorococcus transcriptome were evident within six hours of co-culture, with groups of transcripts changing in different temporal waves. Many transcriptional changes persisted throughout the 48-hour experiment, indicating that the presence of the heterotroph affected a stable shift in Prochlorococcus physiology. These initial transcriptome changes largely correspond to reduced stress conditions within Prochlorococcus, as inferred from decreases in relative abundance for transcripts encoding DNA repair enzymes and many members of the ‘high-light inducible’ family of stress response proteins. Notable changes were also seen in transcripts encoding components of the photosynthetic apparatus (particularly an increase in PSI subunits and chlorophyll synthesis enzymes), ribosomal proteins and biosynthetic enzymes. Changes in secretion-related proteins and transporters also highlight the potential for metabolic exchange between the two strains.
 
Overall design At each of 7 timepoints, samples from 3 biological replicate co-cultures are compared to 3 biological replicate axenic Prochlorococcus cultures that serve as a control.
 
Contributor(s) Biller SJ, Coe A, Chisholm SW
Citation(s) 27258949
Submission date Sep 28, 2015
Last update date May 15, 2019
Contact name Steven Biller
E-mail(s) sbiller@mit.edu
Organization name MIT
Department Civil and Environmental Engineering
Street address 15 Vassar St, MIT 48-424
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platforms (2)
GPL20970 Illumina HiSeq 2000 (Prochlorococcus marinus str. NATL2A)
GPL20972 Illumina HiSeq 2000 (Alteromonas macleodii; Prochlorococcus marinus str. NATL2A)
Samples (42)
GSM1896826 Co-culture replicate 1, 12 hours before heterotroph addition
GSM1896827 Co-culture replicate 2, 12 hours before heterotroph addition
GSM1896828 Co-culture replicate 3, 12 hours before heterotroph addition
Relations
BioProject PRJNA297192
SRA SRP064248

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Supplementary file Size Download File type/resource
GSE73511_co-culture_counts.txt.gz 419.5 Kb (ftp)(http) TXT
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Processed data are available on Series record

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