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Series GSE73295 Query DataSets for GSE73295
Status Public on Sep 20, 2018
Title Gene Expression Differences in MYC and RAS Driven Liver Cancer Transgenic
Organism Mus musculus
Experiment type Expression profiling by array
Summary MYC and RAS signaling are two oncogene pathways important in human liver cancer. We sought to model liver cancer driven by either MYC or RAS using conditional LAP-tTA crossed to either TRE-MYC or TRE-HRAS models in the FVB/N background, and to understand oncogenic signaling pathways that are distinct between each model. Non-tumor tissue, from LAP-tTTA (LT2) mice were used as controls. In this study, we generated tumors from either MYC or RAS driven tumors and compared global gene expression changes to each other and control samples.
 
Overall design LAP-tTA (LT2) control, LAP-tTA x TRE-MYC (MYC), or LAP-tTA x TRE-HRAS (RAS) were bred. Tumor initiation was achieved by removing doxycycline from the diet of dual-transgenic mice to allow for MYC or HRAS oncogene expression. Tumors were collected and flash frozen in liquid nitrogen followed by total RNA isolation and Agilent mRNA array analysis. Four tumors or control tissues from each genotype were analyzed.
 
Contributor(s) Goga A
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Submission date Sep 22, 2015
Last update date Sep 22, 2018
Contact name Andrei Goga
E-mail(s) andrei.goga@ucsf.edu
Organization name UCSF
Department Cell & Tissue Biology
Lab Goga Lab
Street address 513 Parnassus Ave
City San Francisco
State/province CA
ZIP/Postal code 94143-0512
Country USA
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (12)
GSM1890239 Control1
GSM1890240 Control2
GSM1890241 Control3
Relations
BioProject PRJNA296670

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE73295_RAW.tar 186.7 Mb (http)(custom) TAR (of TXT)
GSE73295_matrix_average.txt.gz 830.0 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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