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Status |
Public on Feb 14, 2008 |
Title |
Expression data from MCF7 xenografts |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
In order to help determine the genes involved in resistance of breast cancer to endocrine therapy, we compared global gene expression profiles of tamoxifen-resistant MCF-7 WT xenograft tumors with E2-supplemented tumors. Keywords: two group comparison
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Overall design |
MCF7 xenografts were established in ovariectomized five to six week-old nu/nu athymic nude mice supplemented with 0.25 mg 21 day release estrogen pellets by inoculating subcutaneously (s.c.) 5E-6 cells. When tumors reached the size of 150-200 mm3 (3-5 weeks), the animals were randomly allocated to continued estrogen (E2) and estrogen withdrawal plus tamoxifen citrate. Tumors were harvested for molecular studies when they became resistant to treatment and reached the size of 1000 mm3 (n=7).
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Contributor(s) |
Creighton C, Massarweh S, Schiff R |
Citation(s) |
18245484 |
Submission date |
Mar 20, 2007 |
Last update date |
Mar 25, 2019 |
Contact name |
Chad Creighton |
E-mail(s) |
creighto@bcm.tmc.edu
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Organization name |
Baylor College of Medicine
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Department |
Biostatistics, Ducan Cancer Center
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Street address |
One Baylor Plaza, Mail Stop: BCM305
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City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
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Platforms (1) |
GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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Samples (7)
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GSM176725 |
E2-treated MCF7 xenograft, biological rep1 |
GSM176726 |
E2-treated MCF7 xenograft, biological rep2 |
GSM176727 |
E2-treated MCF7 xenograft, biological rep3 |
GSM176728 |
Tam-treated MCF7 xenograft, biological rep1 |
GSM176729 |
Tam-treated MCF7 xenograft, biological rep2 |
GSM176730 |
Tam-treated MCF7 xenograft, biological rep3 |
GSM176731 |
Tam-treated MCF7 xenograft, biological rep4 |
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Relations |
BioProject |
PRJNA98045 |