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Series GSE73099 Query DataSets for GSE73099
Status Public on Sep 17, 2015
Title Small RNA and High Molecular Weight RNA Deep Sequencing Analysis of miR-17~92 Mimics Transfected HeLa cells
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Transient transfection of miRNA mimics are widely used to alter microRNA expression in cells. The goal of this study is to perform small RNA deep sequencing analysis of HeLa cells transfected with miR-17~92 cluster and address potential source of non-specific effects caused by this approach.
Overall design HeLa cells were cultured as adherent cells. Equal molar concentration of miRNA mimics of miR-17~92 (100nM, 16.7nM each) were transfected and harvedsted at 6h post-transfection for small RNA deep sequencing analysis.
Contributor(s) Jin H, Sabouri‐Ghomi M, Xiao C
Citation(s) 26697058
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 AI087634 The Role of miR-17~92 in Autoimmune Diseases THE SCRIPPS RESEARCH INSTITUTE Xiao
R01 AI089854 Functional Analysis of MicroRNAs in Lymphocyte Development and Immune Tolerance THE SCRIPPS RESEARCH INSTITUTE Xiao
R56 AI110403 Regulation of T Follicular Helper Cell Differentiation by MicroRNAs THE SCRIPPS RESEARCH INSTITUTE Xiao
RC1 CA146299 Deep Sequencing of Small Regulatory RNAs in Diffuse Large B cell Lymphoma THE SCRIPPS RESEARCH INSTITUTE Xiao
Submission date Sep 16, 2015
Last update date May 15, 2019
Contact name Changchun Xiao
Phone (858) 784-7640
Organization name The Scripps Research Institute
Department Immunology & Microbial Science
Lab Changchun Xiao Lab
Street address 10550 North Torrey Pines Road
City La Jolla
State/province California
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (2)
GSM1886350 HeLa miR-17~92 mimics
GSM1886351 HeLa
BioProject PRJNA296037
SRA SRP063862

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Supplementary file Size Download File type/resource
GSE73099_NS60_miRNA_cts.xlsx 16.6 Kb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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