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Series GSE73049 Query DataSets for GSE73049
Status Public on Sep 16, 2015
Title genes regulated by mitoflash
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We found mitoflash has function in reprogramming. So we examined the genes expression after activation of mitoflash during somatic cell reprogramming. There are two ways to improve the mitoflash. First we overexpressed CypD, a mPTP component, to improve the mitoflash. Second we used the durgs, mastoparan and paraquat to increase the mitoflash. The MEF cells were reprogrammed, and treatment with mastoparan, paraquat or overexpressed with CypD. After activation of mitoflash at different times, the cells were collected for RNA-Seq.
Overall design MEF cells were reprogrammed and expressed with CypD and Flag (as control) for 4 days. The cells were collected for RNA-Seq (Samples: Falg-D4, CypD-D4). MEF cells were reprogrammed and treated with mastoparan and paraquat for 8 days. The cells were collected for RNA-Seq (Samples: control-D8, mastoparan-D8 and paraquat-D8).
Contributor(s) deyang y, qinyu c
Citation(s) 26549484
Submission date Sep 15, 2015
Last update date May 15, 2019
Contact name zhongfu ying
Phone 13560418156
Organization name chinese academy of sciences
Department stem cell research
Lab stem cell
Street address kaiyuan
City guangzhou
State/province guangdong
ZIP/Postal code 510530
Country China
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (5)
GSM1881448 control-D8
GSM1881449 mastoparan-D8
GSM1881450 paraquat-D8
BioProject PRJNA296000
SRA SRP063853

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Supplementary file Size Download File type/resource
GSE73049_RAW.tar 3.5 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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