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Status |
Public on Sep 16, 2015 |
Title |
genes regulated by mitoflash |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We found mitoflash has function in reprogramming. So we examined the genes expression after activation of mitoflash during somatic cell reprogramming. There are two ways to improve the mitoflash. First we overexpressed CypD, a mPTP component, to improve the mitoflash. Second we used the durgs, mastoparan and paraquat to increase the mitoflash. The MEF cells were reprogrammed, and treatment with mastoparan, paraquat or overexpressed with CypD. After activation of mitoflash at different times, the cells were collected for RNA-Seq.
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Overall design |
MEF cells were reprogrammed and expressed with CypD and Flag (as control) for 4 days. The cells were collected for RNA-Seq (Samples: Falg-D4, CypD-D4). MEF cells were reprogrammed and treated with mastoparan and paraquat for 8 days. The cells were collected for RNA-Seq (Samples: control-D8, mastoparan-D8 and paraquat-D8).
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Contributor(s) |
deyang y, qinyu c |
Citation(s) |
26549484 |
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Submission date |
Sep 15, 2015 |
Last update date |
May 15, 2019 |
Contact name |
zhongfu ying |
E-mail(s) |
yingzhongfu@163.com
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Phone |
13560418156
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Organization name |
chinese academy of sciences
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Department |
stem cell research
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Lab |
stem cell
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Street address |
kaiyuan
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City |
guangzhou |
State/province |
guangdong |
ZIP/Postal code |
510530 |
Country |
China |
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Platforms (1) |
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Samples (5)
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Relations |
BioProject |
PRJNA296000 |
SRA |
SRP063853 |