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Series GSE72941 Query DataSets for GSE72941
Status Public on Nov 09, 2015
Title RNA-seq analysis reveals profound changes in transcript profiles between siCon- and siH19-transfected myotubes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We used high throughput sequencing to analyze the transcriptional profiling of myotubes. By comparing the transcriptional profiling of myotubes with or without H19 knockdown,1131 genes or transcripts were found at least 1.5-fold up-regulated in siH19 relative to siCon cells, meanwhile 508 genes or transcripts were found at least 1.5-fold down-regulated in siH19 over siCon cells.
Overall design 40h following initiation of differentiation, myotubes were transfected with either control siRNA or siH19. 48h later after transfection, total RNA was extracted for library preparation and RNA-seq analysis to compare trancript profiles between siCon and siH19 cells.
Contributor(s) Zhang N, Carmichael GG, Huang Y
Citation(s) 26687445
Submission date Sep 11, 2015
Last update date May 15, 2019
Contact name Na Zhang
Organization name UConn Health
Department Genetics and Genome Sciences
Lab Gordon G. Carmichael
Street address 400 Farmington Ave
City Farmington
State/province CT
ZIP/Postal code 06030
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM1875029 siCon rep1
GSM1875030 siCon rep2
GSM1875031 siCon rep3
This SubSeries is part of SuperSeries:
GSE73014 Genome-wide DNA-methylation and transcriptome change due to lncRNA H19 knockdown in myotubes
BioProject PRJNA295374
SRA SRP063608

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72941_siCon_vs_siH19_sig_diff_pooled.txt.gz 82.7 Kb (ftp)(http) TXT
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