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Series GSE72905 Query DataSets for GSE72905
Status Public on Sep 11, 2015
Title Cellular and molecular response to ruthenium(II)-arene complex with substituted picolinato ligand in HeLa cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Ruthenium(II)-arene complexes are promising drug candidates for therapy of solid tumors. We have previously shown that ruthenium(II)-arene complex [Ru(η6-p-cymene)(L7)Cl] (RuT7) exerts cytotoxic effects in a panel of cancer cell lines (J. Organomet. Chem. 2014, 749, 343–349). In this study we analyzed in vitro cellular response and performed whole-transcriptome microarray gene expression analysis in HeLa cells in order to understand the cellular and molecular mechanism of response to RuT7 complex. Results on biological action of ruthenium complex obtained on cellular level were reflected on molecular level as well. Analysis of functional categories and signaling and biochemical pathways that are associated with HeLa cells' response to ruthenium complex revealed that RuT7 complex leads HeLa cells through intrinsic (mitochondrial) apoptotic pathway, via indirect DNA damage made by action of reactive oxygen species and through direct DNA binding of RuT7. Performed molecular toxicity analysis has shown that RuT7 has fewer associated toxicity profiles than cisplatin. Altogether these results provide the basis for development of RuT7 in animal and pre-clinical studies as a potential drug candidate.
Overall design We hybridized each condition (control, RuT7 and CDDP) at each time point (12 h and 24 h) in triplicate, for a total of 18 samples.
Contributor(s) Jovanović KK, Tanić M, Ivanović I, Gligorijević N, Dojčinović BP, Lj Tešić Ž, Radulović S
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Submission date Sep 10, 2015
Last update date Nov 27, 2018
Contact name Miljana Tanic
Organization name Institute for Oncology and Radiology of Serbia (IORS)
Department Experimental Oncology
Lab Laboratory of Molecular Genetics
Street address Pasterova 14
City Belgrade
ZIP/Postal code 11000
Country Serbia
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (16)
GSM1874253 cell line_CDDP_12h_1
GSM1874254 cell line_CDDP_24h_1
GSM1874255 cell line_CDDP_24h_2
BioProject PRJNA295298

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72905_RAW.tar 55.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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