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Series GSE72897 Query DataSets for GSE72897
Status Public on Mar 01, 2016
Title EWS-FLI1-induced osteosarcoma model unveiled a crucial role of impaired osteogenic differentiation on osteosarcoma development [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary EWS-FLI1, a multi-functional fusion oncogene, is exclusively detectable in Ewing sarcomas. However, previous studies reported that a subset of osteosarcomas also harbor EWS-ETS family fusion, suggesting that the fusion gene may be involved in the development of a particular type of osteosarcomas. Here using the doxycycline inducible EWS-FLI1 system, we established an EWS-FLI1-dependent osteosarcoma model from murine bone marrow stromal cells. We revealed that the withdrawal of EWS-FLI1 expression enhances the osteogenic differentiation of sarcoma cells, leading to mature bone formation. Taking advantage of induced pluripotent stem cell (iPSC) technology, we also showed that the sarcoma-derived iPSCs with cancer-related genetic abnormalities exhibited the impaired differentiation program of osteogenic lineage irrespective of the EWS-FLI1 expression. Finally, we demonstrated that EWS-FLI1 contributed to in vitro sarcoma development from the sarcoma-iPSCs after osteogenic differentiation. These findings demonstrated that modulating cellular differentiation is fundamental principle of the EWS-FLI1-induced osteosarcoma development. Furthermore, the in vitro cancer model using sarcoma-iPSCs should provide a novel platform for dissecting relationship between cancer genome and cellular differentiation.
 
Overall design Chip-seq in mouse EWS-FLI1-induced osteosarcoma cell lines (SCOS#2 )
 
Contributor(s) Komura S, Itakura F, Yamada Y
Citation(s) 26997645
Submission date Sep 10, 2015
Last update date May 15, 2019
Contact name Yasuhiro Yamada
E-mail(s) yasu@ims.u-tokyo.ac.jp
Organization name University of Tokyo
Department Institute of Medical Science
Lab Division of Stem Cell Pathology
Street address 4-6-1, Shirokanedai, Minato-ku
City Tokyo
ZIP/Postal code 108-8639
Country Japan
 
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (2)
GSM1874186 EWS-FLI1-HA-ChipSeq
GSM1874187 input DNA
This SubSeries is part of SuperSeries:
GSE72898 EWS-FLI1-induced osteosarcoma model unveiled a crucial role of impaired osteogenic differentiation on osteosarcoma development
Relations
BioProject PRJNA295294
SRA SRP063561

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Supplementary file Size Download File type/resource
GSE72897_RAW.tar 40.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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