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Series GSE72851 Query DataSets for GSE72851
Status Public on Feb 25, 2017
Title Intragenic DNA methylation prevents cryptic transcription initiations on gene bodies [ART-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In mammals, DNA methylation occurs mainly at 5mC of CpG dinucleotides. The methylation on the promoter leads to the suppression of gene expression, while the functional role of gene body DNA methylation in highly expressed genes has yet to be clarified. Here, we show that the Dnmt3b-dependent intragenic DNA methylation protects the gene body from RNA Polymerase II (RNA Pol II) spurious entry and cryptic transcription initiation. Using different genome-wide approaches, we demonstrate that loss of Dnmt3b leads to an increase of the RNA Pol II engagement within gene bodies and spurious intragenic transcription initiation events. Furthermore, inhibition of RNA Pol II spurious entry depends on the enzymatic activity of the Dnmt3b recruited by H3K36me3. Thus, elongating RNA Pol II triggers an epigenetic crosstalk that involves SetD2, H3K36me3, Dnmt3b, and DNA methylation to ensure gene transcription initiation fidelity with implications for intragenic hypomethylation in cancer.
Overall design Genome-wide analysis of Dnmt3b role in mouse ESCs using ART-seq.
Contributor(s) Neri F, Oliviero S
Citation(s) 28225755
Submission date Sep 09, 2015
Last update date May 15, 2019
Contact name Francesco Neri
Organization name HuGeF
Street address Via Nizza 52
City Torino
State/province Italy
ZIP/Postal code 10126
Country Italy
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM1873376 ART_seq_KO_rep1
GSM1873377 ART_seq_KO_rep2
GSM1873378 ART_seq_WT_rep1
This SubSeries is part of SuperSeries:
GSE72856 Intragenic DNA methylation prevents cryptic transcription initiations on gene bodies
BioProject PRJNA295208
SRA SRP063525

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Supplementary file Size Download File type/resource
GSE72851_RAW.tar 155.2 Mb (http)(custom) TAR (of BEDGRAPH)
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Raw data are available in SRA
Processed data provided as supplementary file

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