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Series GSE72666 Query DataSets for GSE72666
Status Public on Mar 17, 2016
Title High-throughput sequence data of ChIP performed with anti-SMARCAD1 (ASNA) or anti-CBP actibodies.
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Histone acetylation and nucleosome remodeling play a pivotal role in transcriptional regulation. While histone acetylase and ATP-dependent chromatin remodeling activities have been well characterized, how the two activities are coordinated remains to be uncovered. We discovered ATP-dependent histone H2A acetylation activity in Drosophila nuclear extracts. This activity was column-purified and demonstrated to be composed of CBP and SMARCAD1, which belongs to the Etl1 subfamily of the Snf2 family of helicase-related proteins. SMARCAD1 enhanced acetylation of H2A K5 and K8 by CBP in nucleosomes in an ATP-dependent fashion. Expression array analysis of S2 cells having ectopically expressed SMARCAD1 revealed up-regulated genes. Using native genome templates of these up-regulated genes, we found that SMARCAD1 activates their transcription in vitro. Knockdown analysis of SMARCAD1 and CBP indicated overlapping gene control, and ChIP-seq analysis of these commonly controlled genes showed that CBP is recruited to the promoter prior to SMARCAD1. Moreover, Drosophila genetic experiments demonstrated interaction between SMARCAD1/Etl1 and CBP/nej during development. The interplay between the remodeling activity of SMARCAD1 and histone acetylation by CBP sheds light on chromatin and the genome-integrity network.
Overall design Examination of 2 different protein in 1 cell type.
Contributor(s) Ito T
Citation(s) 26888216
Submission date Sep 02, 2015
Last update date May 15, 2019
Contact name Masamichi Doiguchi
Organization name University of Nagasaki
Street address Sakamoto 1-12-4
City Nagasaki
ZIP/Postal code 852-8523
Country Japan
Platforms (1)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
Samples (3)
GSM1867915 input DNA
GSM1867917 CBP ChIP
BioProject PRJNA294616
SRA SRP063273

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Supplementary file Size Download File type/resource
GSE72666_RAW.tar 760.0 Kb (http)(custom) TAR (of CSV, XLS)
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Raw data are available in SRA
Processed data provided as supplementary file

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