GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE72622 Query DataSets for GSE72622
Status Public on Oct 09, 2015
Title KAP1 Recruitment of the 7SK snRNP to Promoters Enables Transcription Elongation by RNA Polymerase II
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The transition from transcription initiation into elongation at promoters of primary response genes (PRG) in metazoan cells is controlled by inducible transcription factors, which utilize P-TEFb to phosphorylate RNA Polymerase II (Pol II) in response to stimuli. Prior to stimulation, a fraction of P-TEFb is recruited to promoters in a catalytically inactive state bound to the 7SK small nuclear ribonucleoprotein (snRNP). However, it remains unclear how and why the 7SK snRNP is assembled at promoters. Here we report that the transcriptional regulator KAP1 directly recruits the 7SK snRNP to facilitate localized release of P-TEFb, promoting rapid Pol II elongation and PRG synthesis in response to stimuli. Collectively, we have discovered and characterized a novel complex, which we term the KEC, which dictates rapid and robust PRG induction upon stimuli.
Overall design KAP1, 7SK snRNP and Pol II at human genes
Contributor(s) McNamara RP, D'Orso I
Citation(s) 26725010, 26981421
Submission date Sep 02, 2015
Last update date May 15, 2019
Contact name Ivan D'Orso
Phone 214-633-1374
Organization name UT Southwestern Medical Center
Department Microbiology
Lab NL3.110A
Street address 5323 Harry Hines
City Dallas
State/province TX
ZIP/Postal code 75390-9048
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (10)
GSM1866693 Total_Pol_II_ChIP-Seq
GSM1866694 Ser-5P_Pol_II_ChIP-Seq
GSM1866695 KAP1_ChIP-Seq
BioProject PRJNA294503
SRA SRP063087

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72622_RAW.tar 1.6 Gb (http)(custom) TAR (of BIGWIG, BROADPEAK, NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap