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Series GSE7259 Query DataSets for GSE7259
Status Public on Jun 01, 2007
Title Pathway and single gene analysis of Caco-2 cell differentiation by ascorbate-stabilized quercetin
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The aim was to investigate mechanisms contributing to quercetin’s previously described effects on cell-proliferation and -differentiation, which contradicted its proposed anti-carcinogenic potency. In a 10-day experiment, 40 µM quercetin stabilized by 1mM ascorbate reduced Caco-2 differentiation up to 50% (P<0.001). Caco-2 RNA from days 5 and 10, hybridized on HG-U133A2.0 Affymetrix® GeneChips®, showed 1,743 affected genes on both days (P<0.01). All 14 Caco-2 differentiation-associated genes showed decreased expression (P<0.01), including intestinal alkaline phosphatase that was confirmed technically (qRT-PCR) and functionally (enzyme-activity).
The 1,743 genes contributed to 27 affected pathways (P<0.05) categorized under 6 gene ontology (GO) processes, including apoptosis and cell-cycle. Genes within these GO-processes showed fold changes that suggest increased cell-survival and -proliferation. Furthermore, quercetin downregulated expression of genes involved in tumor-suppression and phase II metabolism, and upregulated oncogenes.
Gene expression changes mediated by ascorbate-stabilized quercetin were concordant with those occurring in human colorectal carcinogenesis (≈ 80-90%), but were opposite to those previously described for Caco-2 cells exposed to quercetin in the absence of ascorbate (≈ 75-90%).
In conclusion, gene expression among Caco-2 cells exposed to ascorbate-stabilized quercetin showed mechanisms contrary to what is expected for a cancer-preventive agent. Whether this unexpected in vitro effect is relevant in vivo, remains to be elucidated.
Keywords: Colon cancer; Caco-2; quercetin; differentiation
Overall design Caco-2 cells were harvested on days 5 and 10 post-confluency. Per day, fold changes were calculated as quercetin vs. control.
Contributor(s) Dihal AA, Tilburgs C, van Erk MJ, Rietjens IM, Woutersen RA, Stierum RH
Citation(s) 17639512
Submission date Mar 13, 2007
Last update date Dec 06, 2018
Contact name Ashwin Dihal
Phone +31 (0)30-6944545
Fax +31 (0)30 6944989
Organization name TNO Quality of Life
Department Business Unit Physiologival Sciences
Street address Utrechtseweg 48
City Zeist
ZIP/Postal code 3704 HE
Country Netherlands
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (8)
GSM174819 Quercetin day 05 sample 1
GSM174944 Quercetin day 05 sample 2
GSM174945 Control day 05 sample 1
BioProject PRJNA98015

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Supplementary file Size Download File type/resource
GSE7259_RAW.tar 14.6 Mb (http)(custom) TAR (of CEL, CHP)
Processed data provided as supplementary file

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