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Series GSE72563 Query DataSets for GSE72563
Status Public on Sep 01, 2015
Title Haploinsufficiency in bromodomain containing 2 (Brd2) gene remodels synaptic transmission in female mouse striatum in a sex-specific manner
Organism Mus musculus
Experiment type Expression profiling by array
Summary Our previous work showed that Brd2+/- mouse models the sex specific characteristics of juvenile myoclonic epilepsy, a subsyndrome of idiopathic generalized epilepsy. Here, we investigated a role of Brd2 gene in modulating the genomic fabrics responsible for the glutamatergic, GABAergic, dopaminergic, cholinergic and serotonergic transmission in the striatum of female mice. Agilent two-color gene expression arrays and the genomic fabric paradigm were used to determine whether the expression and networking of genes involved in the synaptic transmission differ between Brd2+/- and wildtype mice and whether these differences are sex-specific. As in Brd+/- male mice we found relatively few regulated genes in Brd2+/-female mice with respect to their wildtype counterparts. However, most of the regulated genes were directly related to control of anxiety and seizures, justifying the role of Brd2 upstream control of these traits. Remarkably, the sets of regulated genes in the two sexes did not overlap, supporting the conclusion of sex-specific control of traits by Brd2.
Overall design Agilent mouse two-color gene expression arrays to profile striata of the prefrontal cortices of wildtype (W) and Brd2+/- (H) mouse female (F) adult C57Bl/6j mice. Differently labeled biological replicates were cohybridized with each array. Results of similarly labeled different conditions were compared then averaged for the two fluorescent labels. For instance: FW1 & FW3 were compared with FH1 & FH3, FW2 & FW4 were compared with FH2 & + FH4, and the results of the comparisons averaged. This design uses 100% of the resources, has a better normlaiztion and allows all possible comparisons among the conditions.
Contributor(s) Iacobas DA, Iacobas S, Veliskova J, Velisek L
Citation Iacobas DA. The Genomic Fabric Perspective on the Transcriptome Between Universal Quantifiers and Personalized Genomic Medicine. Biological Theory, 2016. DOI: 10.1007/s13752-016-0245-3
Submission date Aug 31, 2015
Last update date Jan 19, 2018
Contact name Dumitru Andrei Iacobas
Phone 936-261-9926
Organization name Prairie View A&M University
Department Center for Computational Systems Biology
Lab Personalized Genomics
Street address Ann Preston St
City Prairie View
State/province TX
ZIP/Postal code 77446
Country USA
Platforms (1)
GPL10333 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version)
Samples (8)
GSM1865293 FW1
GSM1865294 FW2
GSM1865295 FW3
BioProject PRJNA294330

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72563_RAW.tar 129.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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