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Series GSE72360 Query DataSets for GSE72360
Status Public on Oct 30, 2015
Title Mapping of 5-hydroxymethylcytosine by selective chemical labeling (SCL-seq) in human naive B cells and in vitro differentiated plasmablasts (P1 cells) [HTS]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary We report the genome-wide mapping of 5hmC in naive B cells and in vitro differentiated plasmablasts (P1 cells). In addition, H3K4me1 and H3K27ac histne marks were mapped in in vitro differentiated plasmablasts from two healthy donors (BN358 and BN369) by ChIP-seq.
 
Overall design Genomic DNA from human primary cells was extracted and submitted to the SCL protocol using the Hydroxymethyl Collector kit from Active Motif. After sequencing on an Illumina HiSeq 2000, reads were mapped to the human hg19 genome. H3K4me1 and H3K27ac were mapped in in vitro differentiated plasmablasts (P1 cells) by ChIP-seq.
 
Contributor(s) Salbert G, Avner S
Citation(s) 26565917
Submission date Aug 25, 2015
Last update date May 15, 2019
Contact name Gilles Salbert
E-mail(s) gilles.salbert@univ-rennes1.fr
Phone +33 223 236 625
Organization name University of Rennes 1
Department Biology
Lab UMR6290 CNRS
Street address Avenue du Général Leclerc
City Rennes
ZIP/Postal code 35042
Country France
 
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (10)
GSM1861003 SCL-seq P1 cells
GSM1861004 SCL-seq naive B cells from lymph node
GSM1861005 SCL-seq naive B cells from blood
This SubSeries is part of SuperSeries:
GSE72498 Cell cycle-dependent reconfiguration of the DNA (hydroxy) methylome during terminal differentiation of human B cells into plasma cells
Relations
BioProject PRJNA293878
SRA SRP062840

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72360_RAW.tar 4.0 Gb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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