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Series GSE72287 Query DataSets for GSE72287
Status Public on Jul 26, 2018
Title Mtf2-PRC2 control of canonical Wnt signaling is required for definitive erythropoiesis. [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary genomic loci or increase enzymatic activity, while PRC2 core proteins are required for complex stability and global levels of trimethylation of histone 3 at lysine 27 (H3K27me3). Here, we demonstrate a role for the classical PRC2 accessory protein Mtf2/Pcl2 in the hematopoietic system that is more akin to that of a core PRC2 protein. Mtf2-/- erythroid progenitors demonstrate markedly decreased core PRC2 protein levels and a global loss of H3K27me3 at promoter-proximal regions. The resulting de-repression of transcriptional and signaling networks blocks definitive erythroid development, culminating in Mtf2-/- embryos dying by e15.5 due to severe anemia. Gene regulatory network (GRN) analysis demonstrated Mtf2 directly regulates Wnt signaling in erythroblasts, leading to activated canonical Wnt signaling in Mtf2-deficient erythroblasts, while chemical inhibition of canonical Wnt signaling rescued Mtf2-deficient erythroblast differentiation in vitro. Using a combination of in vitro, in vivo and systems analyses, we demonstrate that Mtf2 is a critical epigenetic regulator of Wnt signaling during erythropoiesis and recast the role of polycomb accessory proteins in a tissue-specific context.
Overall design ChIP-Seq based binding measurements of H3K4me3, PCL2 and IgG controls for mouse embryonic fetal liver cells; for PCL2 wt and knockout sorted for Ter119 presence/absence
CD71+Ter119-/lo and CD71+Ter119high cell fractions from fetal livers of e14.5 WT and Pcl2-/- embryos were FACS-sorted and crosslinked by formaldehyde. ChIP-Seq was performed for H3K27me3 (and IgG control) in both cell fractions from both genotypes. Pcl2 ChIP-seq and its IgG control was performed on both cell fractions from wild-type embryos.
Contributor(s) Stanford WL, Rothberg JL
Citation(s) 29736258
Submission date Aug 24, 2015
Last update date May 15, 2019
Contact name William Stanford
Phone 613-737-8899
Organization name Ottawa Hospital Research Institute
Lab Stanford
Street address 501 Smyth Road
City Ottawa
State/province Ontario
ZIP/Postal code K1H 8L6
Country Canada
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (10)
GSM1859521 PCL2minus_Ter119neg_H3K27me3
GSM1859522 PCL2minus_Ter119neg_IgG
GSM1859523 PCL2minus_Ter119plus_H3K27me3
This SubSeries is part of SuperSeries:
GSE72288 Mtf2-PRC2 control of canonical Wnt signaling is required for definitive erythropoiesis
BioProject PRJNA293667
SRA SRP062761

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Supplementary file Size Download File type/resource
GSE72287_H3K27me3_NullTer119minus_vs_masked_peaks.xls.gz 27.5 Kb (ftp)(http) XLS
GSE72287_H3K27me3_NullTer119plus_vs_masked_peaks.xls.gz 122.2 Kb (ftp)(http) XLS
GSE72287_H3K27me3_WtTer119minus_vs_masked_peaks.xls.gz 104.0 Kb (ftp)(http) XLS
GSE72287_H3K27me3_WtTer119plus_vs_masked_peaks.xls.gz 96.7 Kb (ftp)(http) XLS
GSE72287_Pcl2_Ter119minus_vs_masked_peaks.xls.gz 600.7 Kb (ftp)(http) XLS
GSE72287_Pcl2_Ter119plus_vs_masked_peaks.xls.gz 111.9 Kb (ftp)(http) XLS
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