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Series GSE72141 Query DataSets for GSE72141
Status Public on Jan 27, 2016
Title GATA3-mediated chromatin reprogramming in breast cancer cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Pioneer transcription factors bind to silent chromatin, and initiate cell fate conversion. One potential pioneer factor, GATA3, is a critical component for multiple cellular programs. GATA3 is of particular interest as it regulates gene expression in breast cancers, and low expression correlates with poor prognosis. Here we demonstrate the pioneering activity of GATA3 utilizing a cellular reprogramming system (the mesenchymal-epithelial transition) in breast cancer cells. During the epithelial transition, GATA3 catalyzes chromatin reprogramming by inducing chromatin opening, active enhancer modifications, and nucleosome remodelling. We determined that the transactivation domain is required for this chromatin reprogramming. Importantly, a mutant lacking the transactivation domain possessed the chromatin binding ability but failed to create open chromatin, suggesting binding alone is not sufficient to induce open chromatin. These data illustrate a fundamental mechanism of GATA3-mediated gene regulation, and provide evidence for a pre-engagement state of closed chromatin bound by a pioneer factor.
Overall design Genome-wide mapping of chromatin state in GATA3-mediated mesenchymal-epithelial transition
Contributor(s) Takaku M, Grimm SA, Takashi S, Paul WA
Citation(s) 26922637, 32811816
Submission date Aug 18, 2015
Last update date Aug 31, 2020
Contact name Paul A Wade
Phone 919-541-3392
Organization name NIEHS
Department Laboratory of Molecular Carcinogenesis
Street address 111 TW Alexander Drive
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
Platforms (3)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL15520 Illumina MiSeq (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (80)
GSM1855964 GATA3_ChIP-seq_SE_rep1
GSM1855965 GATA3_ChIP-seq_SE_rep2
GSM1855966 Input_for_GATA3_ChIP-seq_SE_rep1
BioProject PRJNA293191
SRA SRP062544

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72141_ATAC-seq.Controlcells.merged.bigWig 524.7 Mb (ftp)(http) BIGWIG
GSE72141_ATAC-seq.GATA3cells.merged.bigWig 465.0 Mb (ftp)(http) BIGWIG
GSE72141_ATAC-seq.TA1delcells.merged.bigWig 267.2 Mb (ftp)(http) BIGWIG
GSE72141_BRG1.ChIP-seq.Controlcells.merged.bigWig 562.0 Mb (ftp)(http) BIGWIG
GSE72141_BRG1.ChIP-seq.GATA3cells.merged.bigWig 636.7 Mb (ftp)(http) BIGWIG
GSE72141_BRG1.ChIP-seq.TA1delcells.merged.bigWig 563.4 Mb (ftp)(http) BIGWIG
GSE72141_GATA3.ChIP-seq.GATA3cells.37degrees.merged.bigWig 291.1 Mb (ftp)(http) BIGWIG
GSE72141_GATA3.ChIP-seq.GATA3cells.onice.merged.bigWig 301.8 Mb (ftp)(http) BIGWIG
GSE72141_GATA3.homer.peak.bed.gz 351.3 Kb (ftp)(http) BED
GSE72141_H3K27ac.ChIP-seq.Controlcells.merged.bigWig 436.9 Mb (ftp)(http) BIGWIG
GSE72141_H3K27ac.ChIP-seq.GATA3cells.merged.bigWig 508.3 Mb (ftp)(http) BIGWIG
GSE72141_H3K27ac.ChIP-seq.TA1delcells.merged.bigWig 549.0 Mb (ftp)(http) BIGWIG
GSE72141_H3K4me1.ChIP-seq.Controlcells.merged.bigWig 567.3 Mb (ftp)(http) BIGWIG
GSE72141_H3K4me1.ChIP-seq.GATA3cells.merged.bigWig 619.0 Mb (ftp)(http) BIGWIG
GSE72141_H3K4me1.ChIP-seq.TA1delcells.merged.bigWig 527.1 Mb (ftp)(http) BIGWIG
GSE72141_MNase-seq.Controlcells.Rep1.bigWig 95.3 Mb (ftp)(http) BIGWIG
GSE72141_MNase-seq.Controlcells.Rep2.bigWig 97.9 Mb (ftp)(http) BIGWIG
GSE72141_MNase-seq.Controlcells.Rep3.bigWig 113.2 Mb (ftp)(http) BIGWIG
GSE72141_MNase-seq.GATA3cells.Rep1.bigWig 114.9 Mb (ftp)(http) BIGWIG
GSE72141_MNase-seq.GATA3cells.Rep2.bigWig 90.6 Mb (ftp)(http) BIGWIG
GSE72141_MNase-seq.GATA3cells.Rep3.bigWig 113.4 Mb (ftp)(http) BIGWIG
GSE72141_MNase-seq.TA1delcells.Rep1.bigWig 126.3 Mb (ftp)(http) BIGWIG
GSE72141_MNase-seq.TA1delcells.Rep2.bigWig 111.6 Mb (ftp)(http) BIGWIG
GSE72141_MNase-seq.TA1delcells.Rep3.bigWig 118.6 Mb (ftp)(http) BIGWIG
GSE72141_RNAseq.raw_counts_per_gene.txt.gz 332.4 Kb (ftp)(http) TXT
GSE72141_TA1del.homer.peak.bed.gz 343.3 Kb (ftp)(http) BED
GSE72141_Ty1.ChIP-seq.GATA3cells.merged.bigWig 301.8 Mb (ftp)(http) BIGWIG
GSE72141_Ty1.ChIP-seq.TA1delcells.merged.bigWig 177.1 Mb (ftp)(http) BIGWIG
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