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Series GSE72084 Query DataSets for GSE72084
Status Public on Dec 20, 2016
Title Characterization of chromatin and gene expression changes during fasting in mouse liver [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary During fasting transcriptional programs lead to glucose and ketone production. The major TFs, their crosstalk and the enhancers driving it are unknown. We show that fasting massively reorganizes liver chromatin to expose 'fasting enhancers'. By tracking TF footprints, we implicated the major TFs regulating fuel production by two modules. The ketogenic module is executed by a temporally-organized TF cascade. Conversely, in the gluconeogenic module glucocorticoid receptor (GR) augments cAMP-response element-binding protein (CREB) activity in an enhancer-selective manner. Using genomics and single-molecule tracking, we show that GR enhances the number of bound CREB molecules and the quantity, intensity and accessibility of CREB binding sites; leading to synergistic gene expression and glucose production. Our findings reveal that TFs dynamically and cooperatively respond to environmental signals to restore homeostasis.
Overall design characterization of liver transcription factor binding and histone acetylation during fasting using chromatin immunoprecipitation
Contributor(s) Goldstein I, Baek S, Hager GL
Citation(s) 28031249
Submission date Aug 14, 2015
Last update date Jul 25, 2021
Contact name Songjoon Baek
Organization name NCI / NIH
Department CCR
Street address 41 Library Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (38)
GSM1854432 fed CEBPb ChIP-seq rep#1
GSM1854433 fed CEBPb ChIP-seq rep#2
GSM1854434 fed CEBPb ChIP-seq rep#3
This SubSeries is part of SuperSeries:
GSE72087 Characterization of chromatin and gene expression changes during fasting in mouse liver
BioProject PRJNA292913
SRA SRP062423

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72084_Pooled_GH1160-62_mouse_liver_H3K27Ac_fed_ChIP_mm9_hotspot.csv.gz 841.1 Kb (ftp)(http) CSV
GSE72084_Pooled_GH1163-65_mouse_liver_H3K27Ac_fasted_ChIP_mm9_hotspot.csv.gz 902.5 Kb (ftp)(http) CSV
GSE72084_Pooled_GH1593-95_mouse_liver_H3K27Ac_fasted10hr_ChIP_mm9_hotspot.csv.gz 1.3 Mb (ftp)(http) CSV
GSE72084_RAW.tar 4.0 Gb (http)(custom) TAR (of BEDGRAPH)
GSE72084_hs_RC_ChIP_PMH_pCreb_Glucagon_RC_GH1443xGH1607_FDR0.csv.gz 357.9 Kb (ftp)(http) CSV
GSE72084_hs_RC_ChIP_PMH_pCreb_NT_RC_GH1442xGH1606_FDR0.csv.gz 276.5 Kb (ftp)(http) CSV
GSE72084_hs_RC_mouseliver_ChIP_CREB_NT_RC_GH2245xGH2245_FDR0.csv.gz 1.2 Mb (ftp)(http) CSV
GSE72084_hs_RC_mouseliver_ChIP_CREB_fasted_24_r13_RC_GH2106xGH2108_FDR0.csv.gz 291.3 Kb (ftp)(http) CSV
GSE72084_hs_RC_mouseliver_ChIP_CREB_fed_24_r13_RC_GH2103xGH2105_FDR0.csv.gz 338.4 Kb (ftp)(http) CSV
GSE72084_hs_RC_mouseliver_ChIP_CREB_gc_RC_GH2238xGH2246_FDR0.csv.gz 725.2 Kb (ftp)(http) CSV
GSE72084_hs_RC_mouseliver_ChIP_CREB_gc_cort_RC_GH2239xGH2247_FDR0.csv.gz 1.0 Mb (ftp)(http) CSV
GSE72084_hs_RC_mouseliver_ChIP_GR_cort_gluc_RC_GH2243xGH2249_FDR0.csv.gz 301.9 Kb (ftp)(http) CSV
GSE72084_hs_RC_mouseliver_ChIP_pCREB_fasted_24_r13_RC_GH2112xGH2114_FDR0.csv.gz 174.0 Kb (ftp)(http) CSV
GSE72084_hs_RC_mouseliver_ChIP_pCREB_fed_24_r13_RC_GH2109xGH2111_FDR0.csv.gz 196.1 Kb (ftp)(http) CSV
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Raw data are available in SRA
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