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| Status |
Public on Aug 17, 2015 |
| Title |
Spo11-oligo mapping in Saccharomyces species (S. paradoxus, S. mikatae, S. kudriavzevii) and wild-derived S. cerevisiae strains (YPS128, UWOPS03-461.4) |
| Organisms |
Saccharomyces cerevisiae; Saccharomyces paradoxus; Saccharomyces kudriavzevii; Saccharomyces mikatae |
| Experiment type |
Other
|
| Summary |
The nonrandom distribution of meiotic recombination shapes heredity and genetic diversification. A widely held view is that individual hotspots - favored sites of recombination initiation - are always ephemeral because they evolve rapidly toward extinction. An alternative view, often ignored or dismissed as implausible, predicts conservation of the positions of hotspots if they are chromosomal features under selective constraint, such as gene promoters. Here we empirically test opposite predictions of these theories by comparing genome-wide maps of meiotic recombination initiation from widely divergent species in the Saccharomyces clade. We find that the frequent overlap of hotspots with promoters is true of the species tested and, consequently, hotspot positions are well conserved. Remarkably, however, the relative strength of individual hotspots is also highly conserved, as are larger-scale features of the distribution of recombination initiation. This stability, not predicted by prior models, suggests that the particular shape of the yeast recombination landscape is adaptive, and helps in understanding evolutionary dynamics of recombination in other species.
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| Overall design |
Ten samples total: two biological replicate Spo11-oligo maps of each of the following: wild-derived S. cerevisiae strain YPS128, wild-derived S. cerevisiae UWOPS03-461.4, S. paradoxus YPS138, S. mikatae IFO1815, S. kudriavzevii ZP591
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| Contributor(s) |
Lam I, Keeney S |
| Citation(s) |
26586758 |
| |
| Submission date |
Aug 10, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Isabel Lam |
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Molecular Biology
|
| Lab |
Keeney Lab
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (4)
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| GPL17342 |
Illumina HiSeq 2500 (Saccharomyces cerevisiae) |
| GPL20787 |
Illumina HiSeq 2500 (Saccharomyces paradoxus) |
| GPL20788 |
Illumina HiSeq 2500 (Saccharomyces mikatae) |
| GPL20789 |
Illumina HiSeq 2500 (Saccharomyces kudriavzevii) |
|
| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE71930 |
Non-paradoxical evolutionary stability of the recombination initiation landscape in Saccharomycetes |
|
| Relations |
| BioProject |
PRJNA292494 |
| SRA |
SRP062262 |
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