GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE71862 Query DataSets for GSE71862
Status Public on Sep 02, 2015
Title Differential Gene Expression between MCF10A and MCF7 cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary These RNA-seq data were generated to correlate with genomic interaction data in a related Hi-C analysis. MCF10A is a normal-like mammary epithelial cell line and MCF7 is a transformed estrogen responsive breast cancer cell line derived from a metastatic site; both are commonly used in models of breast cancer progression. Analysis revealed a set of genes related to repression of WNT signalling that were both up-regulated in MCF7 and located in genomic regions that had transitioned from closed to open structure in MCF7.
Overall design RNA-seq of MCF10A and MCF7 cells. 3 replicates each. Sequencing was strand-specific and conducted on ribo-depleted RNA.
Contributor(s) Messier T, Barutcu R, Tye C, Boyd J, Hong D, Stein J, Lian J, Stein G
Citation(s) 26415882
Submission date Aug 07, 2015
Last update date May 15, 2019
Contact name Jonathan AR Gordon
Organization name University of Vermont
Department Biochemistry
Street address 89 Beaumont Ave Given E209
City Burlington
State/province VT
ZIP/Postal code 05405
Country USA
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (6)
GSM1847015 MCF10A_RNA-Seq_R1
GSM1847016 MCF10A_RNA-Seq_R2
GSM1847017 MCF10A_RNA-Seq_R3
BioProject PRJNA292330
SRA SRP062188

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71862_DeSeq2_MCF7_vs_MCF10A_foldchange.txt.gz 902.3 Kb (ftp)(http) TXT
GSE71862_MCF7_MCF10A_RSEM_expectedcounts.txt.gz 534.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap