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Series GSE71812 Query DataSets for GSE71812
Status Public on Jun 13, 2016
Title FUS KO mRNA sequencing and anti-FUS RNA immunoprecipitation sequencing
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The aim of our study is to identify the role of FUS in shaping the transcriptome. RNA-seq of two FUS KO clones was performed and compared to wt; for each, four replicates were sequenced.
RNA molecules associated with the FUS protein were determined by means of a RNA immuno-precipitation, followed by high-throughput sequencing. Total RNA was used as a control.
SH-SY5Y cells were used for both experiments.
Overall design RNA-seq: 4 wt samples, 4 A4 KO samples, 4 A5 KO samples.
RIP-seq: 1 input control sample, 3 anti-FUS IP replicates.
Contributor(s) Ruepp MD, Muehlemann O, Colombo M
Citation(s) 27252488
Submission date Aug 06, 2015
Last update date May 15, 2019
Contact name Oliver Muehlemann
Organization name University of Bern
Street address Freiestrasse 6
City Bern
ZIP/Postal code 3012
Country Switzerland
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (16)
GSM1846256 Input RNA
GSM1846257 FUS RIP 1
GSM1846258 FUS RIP 2
BioProject PRJNA292114
SRA SRP062130

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71812_RAW.tar 3.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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