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Series GSE71797 Query DataSets for GSE71797
Status Public on Feb 09, 2017
Title Genome-wide RNA-sequencing (RNA-seq) of benign and malignant prostate cell lines without and with androgen (R1881) stimulation.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary RNA-seq data were obtained from hTERT immortalized human prostate transit amplifying EP156T cells (+/- 10 nM R1881 for 48 hrs), progeny tumorigenic EPT3-M1 cells recovered from mouse metastatic tumor (+/- 10 nM R1881 for 48 hrs) and the prostate cancer cell lines LNCaP (+/- 10 nM R1881 for 48 hrs), VCaP (+/- 1 nM R1881 for 24 hrs) and 22Rv1 (+/- 1 nM R1881 for 24 hrs) (obtained from the American Type Culture Collection). All cells were either stimulated or not stimulated with the synthetic androgen R1881 (1 or 10 nM for 24 or 48 hrs) prior to lysis (Qiagen miRNeasy minikit lysis buffer) and total RNA purification and DNase treatment.
Overall design Quantification of RNA expression in benign and malignant prostate cells in vitro without and with androgen stimulation
Contributor(s) Kalland K, Olsen J
Citation(s) 27378372, 35203681
Submission date Aug 06, 2015
Last update date Apr 05, 2022
Contact name Biaoyang Lin
Organization name ZheJiang University
Department ZCNI
Lab Systems Biology Division
Street address 866 Yu Hang Tang Road Zi Jin Gang Campus Zhejiang University.
City HangZhou
ZIP/Postal code 310058
Country China
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (10)
GSM1845860 2,4-18 RNA-seq
GSM1845861 2,6-18 RNA-seq
GSM1845862 2,8-18 RNA-seq
BioProject PRJNA292095
SRA SRP062110

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71797_RAW.tar 8.0 Mb (http)(custom) TAR (of FPKM_TRACKING)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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