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Series GSE71518 Query DataSets for GSE71518
Status Public on Oct 02, 2016
Title Liver-resident memory CD8+ T cells form a front-line defense against malaria liver-stage infection.
Organism Mus musculus
Experiment type Expression profiling by array
Summary In recent years, various intervention strategies have reduced malaria morbidity and mortality, but further improvements likely depend upon development of a broadly protective vaccine. To better understand immune requirement for protection, we examined liver-stage immunity after vaccination with irradiated sporozoites, an effective though logistically difficult vaccine. We identified a population of memory CD8+ T cells that expressed the gene signature of tissue-resident memory (Trm) T cells and remained permanently within the liver, where they patrolled the sinusoids. Exploring the requirements for liver Trm cell induction, we showed that by combining dendritic cell-targeted priming with liver inflammation and antigen recognition on hepatocytes, high frequencies of Trm cells could be induced and these cells were essential for protection against malaria sporozoite challenge. Our study highlights the immune potential of liver Trm cells and provides approaches for their selective transfer, expansion or depletion, which may be harnessed to control liver infections or autoimmunity.
Overall design 9 samples were analyzed: 3 replicates of memory CD69+KLRG1low PbT-I CD8+ T cells and 3 replicates of memory CD69-KLRG1+ PbT-I CD8+ T cells isolated from the liver of C57/BL6 mice on day 30 p.i. with irradiated sporozoites; 3 replicates of memory KLRG1+CD69- PbT-I CD8+ T cells (Tem) isolated from the spleen of the same C57/BL6 mice on day 30 p.i. with irradiated sporozoites.
Contributor(s) Fernandez-Ruiz D, Ng WY, Holz L, Ma JZ, Zaid A, Wong YC, Lau LS, Mollard V, Cozijnsen A, Collins N, Li J, Davey GM, Kato Y, Devi S, Skandari R, Pauley M, Manton JH, Godfrey DI, Braun A, Tay SS, Tan PS, Bowen DG, Koch-Nolte F, Rissiek B, Carbone FR, Crabb BS, Lahoud MH, Cockburn I, Mueller SN, Bertolino P, McFadden GI, Caminschi I, Heath WR
Citation(s) 27692609
Submission date Jul 29, 2015
Last update date Jun 02, 2017
Contact name Joel Zhi-Iong Ma
Organization name University of Melbourne
Street address Grattan Street
City Parkville
State/province Victoria
ZIP/Postal code 3010
Country Australia
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (9)
GSM1836066 liver_trm_1
GSM1836067 liver_trm_2
GSM1836068 liver_trm_3
BioProject PRJNA291427

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Supplementary file Size Download File type/resource
GSE71518_RAW.tar 36.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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