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Status |
Public on Mar 01, 2007 |
Title |
Effect of insulin infusion on human skeletal muscle |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
To identify insulin responsive genes in humans, in the first protocol, skeletal muscle biopsies from six non-diabetic subjects were obtained before and after a two-hour of hyperinsulinaemic (infusion rate 40 mU/m2/min) euglycemic clamp. A variable infusion of glucose (180 g/l) enriched with tritiated glucose (100 μCi/500 ml) maintained euglycemia during insulin infusion, with monitoring of plasma glucose concentration every 5 to 10 min during the basal and clamp periods using an automated glucose oxidation method (Glucose Analyzer 2, Beckman Instruments, Fullerton, CA).
In the second protocol, skeletal muscle biopsies from six non-diabetic subjects were obtained before and after a 3-hour hyperinsulinemic (infusion rate 40 mU/m2/min) euglycemic clamp in order to increase the effects of insulin on gene expression. A variable infusion of glucose (180 g/l) was used to maintain euglycemia during insulin infusion with monitoring of plasma glucose concentration every 5 to 10 min using an automated glucose oxidation method (Glucose Analyzer 2, Beckman Instruments, Fullerton, CA). Keywords: dose response
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Overall design |
The muscle biopsies were obtained from the vastus lateralis muscle under local anesthesia before and after hyperinsulinaemic (infusion rate 40 mU/m2/min) euglycemic clamp
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Contributor(s) |
Parikh H, Groop LC, Mootha VK |
Citation(s) |
17472435 |
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Submission date |
Feb 27, 2007 |
Last update date |
Aug 10, 2018 |
Contact name |
Hemang Parikh |
E-mail(s) |
parikhhemang78@gmail.com
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Organization name |
Lund University
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Department |
Department of Clinical Sciences-Diabetes and Endocrinology
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Street address |
Building 91, Plan 12, CRC, Entrance 72, Malmö University Hospital
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City |
Malmö |
ZIP/Postal code |
20502 |
Country |
Sweden |
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Platforms (2) |
GPL80 |
[Hu6800] Affymetrix Human Full Length HuGeneFL Array |
GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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Samples (24)
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Relations |
BioProject |
PRJNA98187 |