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Series GSE71456 Query DataSets for GSE71456
Status Public on Mar 16, 2016
Title Derivation and differentiation of haploid human embryonic stem cells [RNA-Seq 1]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Diploidy is a fundamental genetic feature in mammals, in which haploid cells normally arise only as post-meiotic germ cells that serve to insure a diploid genome upon fertilization. Gamete manipulation has yielded haploid embryonic stem (ES) cells from several mammalian species, but as of yet not from humans. Here we analyzed a large collection of human parthenogenetic ES cell lines originating from haploid oocytes, leading to the successful isolation and maintenance of human ES cell lines with a normal haploid karyotype. Haploid human ES cells exhibited typical pluripotent stem cell characteristics such as self-renewal capacity and a pluripotency-specific molecular signature. Although haploid human ES cells resembled their diploid counterparts, they also displayed distinct properties including differential regulation of X chromosome inactivation and genes involved in oxidative phosphorylation, alongside reduction in absolute gene expression levels and cell size. Intriguingly, we found that a haploid genome is compatible not only with the undifferentiated pluripotent state, but also with differentiated somatic fates representing all three embryonic germ layers, despite a persistent dosage imbalance between the autosomes and X chromosome. We expect that haploid human ES cells will provide novel means for studying human functional genomics, development and evolution.
Overall design RNA sequencing analysis was performed on a total of 15 samples, including haploid and diploid human parthenogenetic embryonic stem cells at different differentiation states and cell cycle phases.
Contributor(s) Sagi I, Egli D, Benvenisty N
Citation(s) 26982723
Submission date Jul 28, 2015
Last update date May 15, 2019
Contact name Ido Sagi
Organization name The Hebrew University of Jerusalem
Department Genetics
Lab Nissim Benvenisty Lab, The Azrieli Center for Stem Cells and Genetic Research
Street address Givat Ram
City Jerusalem
ZIP/Postal code 91904
Country Israel
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (15)
GSM1835011 pES10 h-G1 rep1
GSM1835012 pES10 h-G1 rep2
GSM1835013 pES10 d-G1 rep1
This SubSeries is part of SuperSeries:
GSE71458 Derivation and differentiation of haploid human embryonic stem cells
BioProject PRJNA291275
SRA SRP061707

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Supplementary file Size Download File type/resource
GSE71456_Samples_RPKM.txt.gz 3.4 Mb (ftp)(http) TXT
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