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Series GSE71453 Query DataSets for GSE71453
Status Public on Aug 26, 2016
Title Single cell RNA-seq analysis of sensory neurons reveal diverse injury responses after sciatic nerve transection
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We reported the gene expression analysis of different types of sensory neuron with peripheral nerve transection treatment on single cell level. We found substantial variation between myelinated large diameter neurons and small diameter nonpeptidergic nociceptors, in both terms of regeneration response genes regulation as well as fraction of cells respond to nerve injury.
Overall design Two month old adult CAST/Eij ♂ cross C57BL/6♀F1 and C57BL/6 mice were performed sciatic nerve transection on the right side. Single DRG soma from L3-5 on both side were manually picked in the lowest possible volume and then performed single cell RNA-seq using smart-seq2 protocol. A total of 106 dissociated DRG neurons for each side with almost equal number of small, medium and large diameter neurons were lysed and split into three parts in order to constructed bulk cell control.
Contributor(s) Hu G
Citation(s) 27558660
Submission date Jul 28, 2015
Last update date May 15, 2019
Contact name Ganlu Hu
Phone +8613636618854
Organization name University of California, Los Angeles
Department human genetics
Lab Guoping Fan lab
Street address 695 Charles E Young Dr. South
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (123)
GSM1834927 DRG_bulk_Nor_1
GSM1834928 DRG_bulk_Nor_2
GSM1834929 DRG_bulk_Nor_3
BioProject PRJNA291276
SRA SRP061708

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Supplementary file Size Download File type/resource
GSE71453_DRG_SNT_3-7days_TPM_geo.csv.gz 2.4 Mb (ftp)(http) CSV
GSE71453_DRG_SNT_3-7days_TPM_remove_outliers_after_filters_geo.txt.gz 2.1 Mb (ftp)(http) TXT
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Processed data are available on Series record

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